A novel biosensor based on L-homocysteine desulfhydrase enzyme immobilized in eggshell membrane
Küçük Resim Yok
Tarih
2007
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Bv
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A novel biosensor for homocysteine determination has been developed. The biosensor was fabricated with L-homocysteine clesulfhydrase immobilized on the ammonium selective electrode by means of eggshell membrane. The measurement principle is based on determination of ammonia due to the enzymatic reaction in the medium by ammonium selective electrode. The effects of enzyme loading, glutaraldehyde concentration, pH, buffer concentration, temperature, dithiotreitol (DTT) concentration and ionic strength adjustment buffer (ISA) on the biosensor response were investigated in detail. The linear detection range and limit of detection (LOD) for homocysteine were found to be 0.15 - 1.8 mM and 55 mu M, respectively. Finally, the homocysteine biosensor has been applied to plasma samples for determination of total homocysteine contents. (c) 2007 Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
homocysteine biosensor, L-homocysteine desulfhydrase, eggshell membrane, ammonium selective electrode
Kaynak
Journal of Molecular Catalysis B-Enzymatic
WoS Q Değeri
Q2
Scopus Q Değeri
N/A
Cilt
49
Sayı
01.Apr
