Toxoplasma gondii destroys Her2/Neu-expressing mammary cancer cells in vitro using a continuous feed medium approach

Basit Öğe Kaydı
dc.contributor.authorAtalay Şahar, E.
dc.contributor.authorDöşkaya, M.
dc.contributor.authorKarakavuk, M.
dc.contributor.authorCan, H.
dc.contributor.authorGül, A.
dc.contributor.authorGürüz, A.Y.
dc.contributor.authorYeniay, L.
dc.date.accessioned2020-12-01T11:52:27Z
dc.date.available2020-12-01T11:52:27Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractINTRODUCTION: Toxoplasma gondii is an opportunistic protozoan and can be grown using several human cell lines. Breast cancer is the second leading cause of cancer death in women. Her2/Neu-expressing mammary cancer cell lines called TUBO can be grown in vitro. In recent years, protozoan parasites have become popular means of use in cancer therapy research. In this study, we analyzed whether T. gondii tachyzoites can destroy TUBO cells using a novel continuous feed medium approach. METHODOLOGY: Two sets of flasks (each containing four groups) containing TUBO cells were inoculated with T. gondii Ankara strain tachyzoites. First set containing 5×106 TUBO cells were inoculated with TUBO-tachyzoite ratios of 1:2, 1:1, 2:1, and 4:1 and second set containing 1×106 TUBO cells were inoculated with TUBO-tachyzoite ratios of 10:1, 100:1, 1000:1, and 2000:1. Thereafter, culture supernatants were harvested at various days until TUBO cells were destroyed and tachyzoites were counted. RESULTS: In the first and second sets of flasks, TUBO cells were destroyed between days 8 to 12 and 12 to 25, respectively. In addition, the amount of tachyzoites increased 7- 43 and 595 to 112500 times in the first and second set of flasks, respectively. CONCLUSIONS: These results show that T. gondii tachyzoites successfully destroy Her2/Neu-expressing mammary cancer cells using a continuous feed medium approach. Although this idea may be too premature for the moment, the approach defined herein may support future researchers investigating the relationship between cancer and parasites which can make important progress toward saving cancer patient lives. Copyright (c) 2020 Esra Atalay Sahar, Mert Doskaya, Muhammet Karakavuk, Huseyin Can, Aytul Gul, Adnan Yuksel Guruz, Aysu Degirmenci Doskaya, Levent Yeniay.en_US
dc.identifier.doi10.3855/jidc.12820en_US
dc.identifier.endpage1209en_US
dc.identifier.issn1972-2680
dc.identifier.issue10en_US
dc.identifier.pmid33175718en_US
dc.identifier.scopus2-s2.0-85096081369en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1204en_US
dc.identifier.urihttps://doi.org/10.3855/jidc.12820
dc.identifier.urihttps://hdl.handle.net/11454/61542
dc.identifier.volume14en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherNLM (Medline)en_US
dc.relation.ispartofJournal of infection in developing countriesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectbreast canceren_US
dc.subjectcanceren_US
dc.subjectHer2/Neuen_US
dc.subjectin vitroen_US
dc.subjectToxoplasma gondiien_US
dc.subjectTUBOen_US
dc.titleToxoplasma gondii destroys Her2/Neu-expressing mammary cancer cells in vitro using a continuous feed medium approachen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu