Graphene oxide incorporated polystyrene electrospun nanofibers for immunosensing of CD36 as a marker of diabetic plasma

Basit Öğe Kaydı
dc.authoridER ZEYBEKLER, Simge/0000-0002-5814-0618
dc.authorscopusid57222095969
dc.authorscopusid57219316379
dc.authorwosidER, Simge/HGU-2032-2022
dc.contributor.authorEr, Simge
dc.contributor.authorDemirkol, Dilek Odaci
dc.date.accessioned2023-01-12T19:54:28Z
dc.date.available2023-01-12T19:54:28Z
dc.date.issued2022
dc.departmentN/A/Departmenten_US
dc.description.abstractElectrospun nanofibers (ESNF) offer us a chance to obtain nanoscale building blocks by adding the desired modification agent to the polymer solution. Here, nanocomposite-based electrospun nanofibers designed for the recognition surface of the developed immunosensor were used for the first time in the determination of CD36. Firstly, graphene oxide (GO) was synthesized from graphite powder (GR), and GO sheets were silanized with different amounts of (3-Aminopropyl)triethoxysilane (APTES). Synthesized GO-APTES nanocomposite and polystyrene (PS) solution were mixed in different ratios to obtain uniform nanofibers without beads. As a result of the amino groups obtained on the surface of the nanofibers, the surface was made ready for covalent immobilization of the Anti-CD36 antibody. The nanofibers obtained under the optimum conditions determined were deposited on the surface of the screen-printed carbon electrode (SPCE) by the electrospinning technique. Then, Anti-CD36 was immobilized on the PS/GO-APTES modified SPCE through covalent bonding and used to prepare the biofunctional surface for the usage of bioelectrochemistry of CD36. The optimum Anti-CD36 con-centration decided to be used in experiments was determined as 10 mu g/mL. The linear detection range of CD36 was from 0.5 to 20 ng/mL, and the detection limit was 0.999 ng/mL. Finally, the developed PS/GO-APTES/Anti-CD36 immunosensor was used for the determination of CD36 in artificial blood serum without any interference effect.en_US
dc.description.sponsorshipCouncil of Higher Education of Turkey [YOK 100/2000]; Ege University Scientific Research Projects Coordination Unit [FGA-2021-22688]en_US
dc.description.sponsorshipS. ER who is supported in the special subject (Sensor Technologies) thanks The Council of Higher Education of Turkey 100/2000 PhD Project (YOK 100/2000 PhD Project). This study is supported by Ege University Scientific Research Projects Coordination Unit (Project Number: FGA-2021-22688).en_US
dc.identifier.doi10.1016/j.bioelechem.2022.108083
dc.identifier.issn1567-5394
dc.identifier.issn1878-562X
dc.identifier.pmid35150998en_US
dc.identifier.scopus2-s2.0-85124191108en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.bioelechem.2022.108083
dc.identifier.urihttps://hdl.handle.net/11454/76431
dc.identifier.volume145en_US
dc.identifier.wosWOS:000790444600001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Saen_US
dc.relation.ispartofBioelectrochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGraphene oxideen_US
dc.subjectPolystyreneen_US
dc.subjectElectrospun nanofibersen_US
dc.subjectElectrospinningen_US
dc.subjectImmunosensingen_US
dc.subjectSilaneen_US
dc.subjectOrientationen_US
dc.subjectSilanizationen_US
dc.subjectStabilityen_US
dc.subjectBehavioren_US
dc.titleGraphene oxide incorporated polystyrene electrospun nanofibers for immunosensing of CD36 as a marker of diabetic plasmaen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu