First report of a novel 108 bp deletion and five novel SNPs in PRNP gene of stray cats and in silico analysis of their possible relation with feline spongiform encephalopathy

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dc.authoridErkunt Alak, Sedef/0000-0001-8563-1239
dc.contributor.authorGuvendi, Mervenur
dc.contributor.authorCan, Huseyin
dc.contributor.authorKoseog, Ahmet Efe
dc.contributor.authorAlak, Sedef Erkunt
dc.contributor.authorUn, Cemal
dc.date.accessioned2024-08-31T07:50:11Z
dc.date.available2024-08-31T07:50:11Z
dc.date.issued2024
dc.departmentEge Üniversitesien_US
dc.description.abstractPrion diseases are fatal neurodegenerative diseases affecting humans and animals. A relationship between variations in the prion gene of some species and susceptibility to prion diseases has been detected. However, variations in the prion protein of cats that have close contact with humans and their effect on prion protein are not well-known. Therefore, this study aimed to investigate the variations of prion protein-encoding gene (PRNP gene) in stray cats and to evaluate variants detected in terms of genetic factors associated with susceptibility or resistance to feline spongiform encephalopathy using bioinformatics tools. For this, cat DNA samples were amplified by a PCR targeting PRNP gene and then sequenced to reveal the variations. Finally, the effects of variants on prion protein were predicted by bioinformatics tools. According to the obtained results, a novel 108 bp deletion and nine SNPs were detected. Among SNPs, five (c314A>G, c.454T>A, c.579G>C, c.642G>C and c.672G>C) were detected for the first time in this study. Bioinformatics findings showed that c.579G>C (Q193H), c.454T>A (Y152N) and c.457G>A (E153K) variants have deleterious effects on prion protein and c.579G>C (Q193H) has high amyloid propensities. This study demonstrates prion protein variants of stray cats and their deleterious effects on prion protein for the first time.en_US
dc.identifier.doi10.1016/j.tcam.2024.100859
dc.identifier.issn1938-9736
dc.identifier.issn1946-9837
dc.identifier.pmid38508487en_US
dc.identifier.scopus2-s2.0-85189537020en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.tcam.2024.100859
dc.identifier.urihttps://hdl.handle.net/11454/105129
dc.identifier.volume59en_US
dc.identifier.wosWOS:001223195300001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherW B Saunders Co-Elsevier Incen_US
dc.relation.ispartofTopics In Companion Animal Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240831_Uen_US
dc.subjectPrnp Geneen_US
dc.subjectFseen_US
dc.subjectSequencingen_US
dc.subjectSnpen_US
dc.subjectDeletionen_US
dc.subjectStray Caten_US
dc.titleFirst report of a novel 108 bp deletion and five novel SNPs in PRNP gene of stray cats and in silico analysis of their possible relation with feline spongiform encephalopathyen_US
dc.typeArticleen_US

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