Cytotoxic responses of carnosic acid and doxorubicin on breast cancer cells in butterfly-shaped microchips in comparison to 2D and 3D culture

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dc.contributor.authorYildiz-Ozturk, Ece
dc.contributor.authorGulce-Iz, Sultan
dc.contributor.authorAnil, Muge
dc.contributor.authorYesil-Celiktas, Ozlem
dc.date.accessioned2019-10-27T11:09:13Z
dc.date.available2019-10-27T11:09:13Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractTwo dimensional (2D) cell culture systems lack the ability to mimic in vivo conditions resulting in limitations for preclinical cell-based drug and toxicity screening assays and modelling tumor biology. Alternatively, 3D cell culture systems mimic the specificity of native tissue with better physiological integrity. In this regard, microfluidic chips have gained wide applicability for in vitro 3D cancer cell studies. The aim of this research was to develop a 3D biomimetic model comprising culture of breast cancer cells in butterfly-shaped microchip to determine the cytotoxicity of carnosic acid and doxorubicin on both estrogen dependent (MCF-7) and independent (MDAMB231) breast cancer cells along with healthy mammary epithelial cells (MCF-10A) in 2D, 3D Matrigel (TM) and butterfly-shaped microchip environment. According to the developed mimetic model, carnosic acid exhibited a higher cytotoxicity towards MDAMB 231, while doxorubicin was more effective against MCF-7. Although the cell viabilities were higher in comparison to 2D and 3D cell culture systems, the responses of the investigated molecules were different in the microchips based on the molecular weight and structural complexity indicating the importance of biomimicry in a physiologically relevant matrix.en_US
dc.identifier.doi10.1007/s10616-016-0062-3en_US
dc.identifier.endpage347en_US
dc.identifier.issn0920-9069
dc.identifier.issn1573-0778
dc.identifier.issue2en_US
dc.identifier.pmid28191587en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage337en_US
dc.identifier.urihttps://doi.org/10.1007/s10616-016-0062-3
dc.identifier.urihttps://hdl.handle.net/11454/32265
dc.identifier.volume69en_US
dc.identifier.wosWOS:000398104900012en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofCytotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMicrofluidicsen_US
dc.subjectMicrochipen_US
dc.subjectBreast canceren_US
dc.subjectCarnosic aciden_US
dc.subjectDoxorubicinen_US
dc.titleCytotoxic responses of carnosic acid and doxorubicin on breast cancer cells in butterfly-shaped microchips in comparison to 2D and 3D cultureen_US
dc.typeArticleen_US

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