Offline glucose biomonitoring in yeast culture by polyamidoamine/ cysteamine-modified gold electrodes

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dc.contributor.authorYuksel M.
dc.contributor.authorAkin M.
dc.contributor.authorGeyik C.
dc.contributor.authorDemirkol D.O.
dc.contributor.authorOzdemir C.
dc.contributor.authorBluma A.
dc.contributor.authorHöpfner T.
dc.contributor.authorBeutel S.
dc.contributor.authorTimur S.
dc.contributor.authorScheper T.
dc.date.accessioned2019-10-27T08:34:00Z
dc.date.available2019-10-27T08:34:00Z
dc.date.issued2011
dc.departmentEge Üniversitesien_US
dc.description.abstractThis article deals with the use of pyranose oxidase (PyOx) and glucose oxidase (GOx) enzymes in amperometric biosensor design and their application in monitoring fermentation processes with the combination of flow injection analysis (FIA). The amperometric studies were carried out at -0.7 V by following the oxygen consumption due to the enzymatic reactions for both batch and FIA modes. Optimization studies (enzyme amounts and pH) and analytical parameters such as linearity, repeatability, effect of interference, storage, and operational stabilities have been studied. Under optimized conditions, for the PyOx-based biosensor, linear graph was obtained from 0.025 to 0.5 mM glucose in phosphate buffer (50 mM) at pH 7.0 with the equation of y = 3.358x + 0.028 and R2 = 0.998. Linearity was found to be 0.01-1.0 mM in citrate buffer (50 mM and pH 4.0) with the equation of y = 1.539x + 0.181 and R2 = 0.992 for the GOx biosensor. Finally, these biosensor configurations were further evaluated in a conventional flow injection system. Results from batch experiments provide a guide to design sensitive, stable, and interference-free biosensors for FIA mode. Biosensor stability, dynamic range, and repeatability were also studied in FIA conditions, and the applicability for the determination of glucose in fermentation medium could be successfully demonstrated. The FIA-combined glucose biosensor was used for the offline monitoring of yeast fermentation. The obtained results correlated well with HPLC measurements. © 2011 American Institute of Chemical Engineers.en_US
dc.identifier.doi10.1002/btpr.544en_US
dc.identifier.endpage538en_US
dc.identifier.issn8756-7938
dc.identifier.issue2en_US
dc.identifier.pmid21485034en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage530en_US
dc.identifier.urihttps://doi.org/10.1002/btpr.544
dc.identifier.urihttps://hdl.handle.net/11454/26946
dc.identifier.volume27en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofBiotechnology Progressen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFlow injection analysisen_US
dc.subjectGlucose biomonitoringen_US
dc.subjectGlucose oxidaseen_US
dc.subjectPyranose oxidaseen_US
dc.subjectSelf-assembled monolayersen_US
dc.titleOffline glucose biomonitoring in yeast culture by polyamidoamine/ cysteamine-modified gold electrodesen_US
dc.typeArticleen_US

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