Determination of T-cell clonality and expression profiles of Toll-like receptors signaling pathway genes and related miRNAs in patients with mycosis fungoides

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dc.authorscopusid56499294100
dc.authorscopusid24066601700
dc.authorscopusid6603112460
dc.authorscopusid15750610300
dc.authorscopusid6505834493
dc.authorscopusid6507893937
dc.contributor.authorSeçme, M.
dc.contributor.authorDodurga, Y.
dc.contributor.authorDemirkan, N.Ç.
dc.contributor.authorKaçar, N.
dc.contributor.authorGünel, N.S.
dc.contributor.authorAçıkbaş, İ.
dc.date.accessioned2024-08-25T18:36:15Z
dc.date.available2024-08-25T18:36:15Z
dc.date.issued2024
dc.departmentEge Üniversitesien_US
dc.description.abstractCutaneous T-cell lymphomas (CTCL) encompass a group of diseases characterized by the presence of malignant clonal CD4+ T lymphocytes in the skin. Mycosis fungoides (MF) is the most prevalent form of CTCL, accounting for approximately 60 % of cutaneous T-cell lymphomas and 50 % of all primary cutaneous lymphomas. Despite ongoing research, the precise pathogenesis of MF remains incompletely understood. Toll-like receptors (TLRs) have the ability to specifically recognize ligands, subsequently induce the expression of diverse genes and activate innate immunity within the cell. Furthermore, miRNAs play a crucial role in regulating various aspects of immune cell function. The aim of our study was to explore the potential roles of TLRs and the genes implicated in their signal transduction, along with the expression status of miRNAs in the mechanisms underlying MF. Additionally, we assessed the clonal status and compared it with clinicopathological data using a T-cell clonality assay. To determine the expression status of TLR pathway genes and miRNAs, we conducted RT-PCR analysis on 52 MF samples and 50 control paraffin block materials. Pathway analysis were conducted using the KEGG database. T-cell receptor (TCR) gamma clonality changes were evaluated. Results from the study revealed increased expressions of TLR-1, -4, -8, IRF7, TRAF3, MEK1, MEK2, Elk1, NFkB, hsa-miR-21-5p, and hsa-miR-155-5p, as well as decreased expressions of hsa-miR-130a-3p, hsa-miR-210-3p, and hsa-let-7e-5p in the MF group. TCR gamma clonal change analysis demonstrated that 55.5 % of the analysed DNAs exhibited monoclonal and biallelic patterns, while 45.5 % displayed polyclonality. These findings collectively suggest the potential influence and therapeutic possibilities of the TLR signalling pathway in the molecular pathogenesis of MF. © 2023 Elsevier B.V.en_US
dc.description.sponsorshipPamukkale Üniversitesi, PAÜ: 2018SABE004en_US
dc.description.sponsorshipFunding: This research was produced from the PhD thesis of M.S and supported by the Scientific Research Coordinatorship of Pamukkale University as the project numbered as 2018SABE004).en_US
dc.identifier.doi10.1016/j.gene.2023.147825
dc.identifier.issn0378-1119
dc.identifier.pmid37748629en_US
dc.identifier.scopus2-s2.0-85172681862en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.gene.2023.147825
dc.identifier.urihttps://hdl.handle.net/11454/100574
dc.identifier.volume891en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier B.V.en_US
dc.relation.ispartofGeneen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240825_Gen_US
dc.subjectClonalityen_US
dc.subjectmiRNAsen_US
dc.subjectMycosis fungoidesen_US
dc.subjectToll-like receptorsen_US
dc.subjectDNAen_US
dc.subjectimmunoglobulin enhancer binding proteinen_US
dc.subjectinterferon regulatory factor 7en_US
dc.subjectmicroRNAen_US
dc.subjectmicroRNA 130a 3pen_US
dc.subjectmicroRNA 146a 5pen_US
dc.subjectmicroRNA 155en_US
dc.subjectmicroRNA 155 5pen_US
dc.subjectmicroRNA 200a 3pen_US
dc.subjectmicroRNA 204 5pen_US
dc.subjectmicroRNA 21en_US
dc.subjectmicroRNA 21 5pen_US
dc.subjectmicroRNA 210 3pen_US
dc.subjectmicroRNA 214 3pen_US
dc.subjectmicroRNA 223 3pen_US
dc.subjectmicroRNA 224 5pen_US
dc.subjectmicroRNA 375en_US
dc.subjectmicroRNA 424 5pen_US
dc.subjectmicroRNA let 7e 5pen_US
dc.subjectmitogen activated protein kinase kinase 1en_US
dc.subjectmitogen activated protein kinase kinase 2en_US
dc.subjectparaffinen_US
dc.subjecttoll like receptoren_US
dc.subjecttoll like receptor 1en_US
dc.subjecttoll like receptor 4en_US
dc.subjecttoll like receptor 8en_US
dc.subjecttranscription factor Elk 1en_US
dc.subjecttumor necrosis factor receptor associated factor 3en_US
dc.subjectunclassified drugen_US
dc.subjectadolescenten_US
dc.subjectadulten_US
dc.subjectageden_US
dc.subjectalleleen_US
dc.subjectArticleen_US
dc.subjectchilden_US
dc.subjectclonal variationen_US
dc.subjectcontrolled studyen_US
dc.subjectDNA determinationen_US
dc.subjectElk1 geneen_US
dc.subjectERK1 geneen_US
dc.subjectERK2 geneen_US
dc.subjectfemaleen_US
dc.subjectgene expressionen_US
dc.subjectgene functionen_US
dc.subjecthistopathologyen_US
dc.subjecthumanen_US
dc.subjecthuman cellen_US
dc.subjecthuman tissueen_US
dc.subjectinfanten_US
dc.subjectIRAK 4 geneen_US
dc.subjectIRAK1 geneen_US
dc.subjectIRF 7 geneen_US
dc.subjectKEGGen_US
dc.subjectmajor clinical studyen_US
dc.subjectmaleen_US
dc.subjectMEK1 geneen_US
dc.subjectMEK2 geneen_US
dc.subjectmolecular pathologyen_US
dc.subjectmycosis fungoidesen_US
dc.subjectMyD88 geneen_US
dc.subjectNfkappaB geneen_US
dc.subjectpathway analysisen_US
dc.subjectprotein expressionen_US
dc.subjectprotein functionen_US
dc.subjectreal time polymerase chain reactionen_US
dc.subjectsignal transductionen_US
dc.subjectT lymphocyteen_US
dc.subjectTAB2 geneen_US
dc.subjectTIRAP geneen_US
dc.subjectTLR 1 geneen_US
dc.subjectTLR 10 geneen_US
dc.subjectTLR 2 geneen_US
dc.subjectTLR 3 geneen_US
dc.subjectTLR 4 geneen_US
dc.subjectTLR 5 geneen_US
dc.subjectTLR 6 geneen_US
dc.subjectTLR 7 geneen_US
dc.subjectTLR 8 geneen_US
dc.subjectTLR 9 geneen_US
dc.subjectTLR geneen_US
dc.subjectTLR signalingen_US
dc.subjectTRAF 3 geneen_US
dc.subjectTRAF 6 geneen_US
dc.subjectTRIF geneen_US
dc.subjectvery elderlyen_US
dc.titleDetermination of T-cell clonality and expression profiles of Toll-like receptors signaling pathway genes and related miRNAs in patients with mycosis fungoidesen_US
dc.typeArticleen_US

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