Low night temperature tolerance determining traits correlate with paraquat tolerance in Grapevine genotypes
Küçük Resim Yok
Tarih
2010
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, dört asma (Vitis vinifera L.) genotipinde düşük gece sıcaklığına (DGS) toleransı belirleyen karakterler ile paraquat (PQ) toleransı arasındaki ilişki ortaya koyulmuştur. Asma genotipleri sera koşullarında yetiştirilerek, $5^ oC$ DGS’na maruz bırakılmıştır. Seçilen genotipler arasında DGS’na tepkide temel farklılıklar, yapraklardaki elektrolit sızıntısı ve lipid peroksidasyonu ile belirlenmiştir. Yapraktaki hasar oranına göre, Dimrit ve Razakı genotipleri DGS’na toleranslı, Hatun Parmağı ve Ata Sarısı ise duyarlı genotipler olarak bulunmuştur. DGS’na tolerant genotipler, duyarlı genotiplere göre PQ’a daha fazla tolerans göstermişlerdir. Tolerant ve duyarlı genotiplerin her ikisinde de, ışık hasarı indikatörü PSII aktivitesi sabit kalmıştır. Düşük sıcaklık uygulanan toleranslı genotiplerde fotosentetik pigment içerikleri korunurken, duyarlı genotiplerde, Hatun Parmağı ve Ata Sarısı, total klorofil (Kl) içeriğinde yaklaşık % 48 ve 49, karotenoid (Kar) içeriğinde ise yaklaşık % 51ve 67 önemli kayıp ölçülmüştür. Duyarlı genotiplerin çözünür protein içeriklerinde değişiklik olmazken, serbest prolin miktarları meydana gelen hasara tepki olarak hızla artmıştır. Sonuçların analizi, membran hasarının göstergesi olan lipid peroksidasyon düzeyi ile çözünür protein içeriği arasında pozitif bir korelasyon, PQ toleransı, Kl/Kar oranı, klorofil ve karotenoid içeriği arasında ise negatif korelasyon bulunduğunu göstermiştir. Bulgularımız, asma genotiplerinde DGS’na toleransın, fotosentetik mekanizma ve membranların oksidatif stresten daha iyi korunması temelinde olduğunu ortaya koymaktadır.
The objective of this study was to reveal that low night temperature (LNT) tolerance concerning traits and relation with paraquat (PQ) tolerance in four grapevine (Vitis vinifera L.) genotypes. Grapevine genotypes were grown in greenhouse conditions and subjected to LNT at $5^ oC$. Electrolyte leakage and lipid peroxidation of leaves indicates fundamental differences between the selected genotypes regarding their response to LNT stress. According to extent of damage, Dimrit and Razakı were assessed as tolerant, while Hatun Parmağı and Ata Sarısı were sensitive to LNT. Low night temperature tolerant genotypes, showed higher resistance to PQ as compared to sensitive genotypes. Photosytem II activity as an indicator of photodamage maintained almost stable in both tolerant and sensitive genotypes. in tolerant genotypes photosynthetic pigment contents was protected, whereas in sensitive genotypes, Hatun Parmağı and Ata Sarısı, significant losses occurred; in total chlorophyll (Chl) content about 48 and 49 % and in carotenoid (Car) content about 51 and 67 % when plants subjected to chilling. in sensitive genotypes, soluble protein content was remained unchanged, free proline content was sharply increased in a response of damage. the correlation analysis showed that lipid peroxidation level indicating membrane damage is having positive correlation with soluble protein content and negatively correlated with, PQ tolerance, Chl/Car ratio, chlorophyll and carotenoid content. This data confirmed the LNT tolerance of grapevine genotypes mainly based on better protection of photosynthetic apparatus and membranes from oxidative stress.
The objective of this study was to reveal that low night temperature (LNT) tolerance concerning traits and relation with paraquat (PQ) tolerance in four grapevine (Vitis vinifera L.) genotypes. Grapevine genotypes were grown in greenhouse conditions and subjected to LNT at $5^ oC$. Electrolyte leakage and lipid peroxidation of leaves indicates fundamental differences between the selected genotypes regarding their response to LNT stress. According to extent of damage, Dimrit and Razakı were assessed as tolerant, while Hatun Parmağı and Ata Sarısı were sensitive to LNT. Low night temperature tolerant genotypes, showed higher resistance to PQ as compared to sensitive genotypes. Photosytem II activity as an indicator of photodamage maintained almost stable in both tolerant and sensitive genotypes. in tolerant genotypes photosynthetic pigment contents was protected, whereas in sensitive genotypes, Hatun Parmağı and Ata Sarısı, significant losses occurred; in total chlorophyll (Chl) content about 48 and 49 % and in carotenoid (Car) content about 51 and 67 % when plants subjected to chilling. in sensitive genotypes, soluble protein content was remained unchanged, free proline content was sharply increased in a response of damage. the correlation analysis showed that lipid peroxidation level indicating membrane damage is having positive correlation with soluble protein content and negatively correlated with, PQ tolerance, Chl/Car ratio, chlorophyll and carotenoid content. This data confirmed the LNT tolerance of grapevine genotypes mainly based on better protection of photosynthetic apparatus and membranes from oxidative stress.
Açıklama
Anahtar Kelimeler
Ziraat Mühendisliği
Kaynak
Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi
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Cilt
20
Sayı
3
