Optimization of chitosanase production by Bacillus mojavensis EGE-B-5.2i

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dc.contributor.authorLiaqat, Fakhra
dc.contributor.authorBahadir, Pinar Soezer
dc.contributor.authorElibol, Murat
dc.contributor.authorEltem, Rengin
dc.date.accessioned2019-10-27T10:01:59Z
dc.date.available2019-10-27T10:01:59Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractMaximum production of industrially important enzymes such as chitosanases through media optimization still holds foremost interest. The present study was conducted to improve chitosanase activity of an indigenous strain identified as Bacillus mojavensis. Initially, carbon and nitrogen sources were optimized by one-variable-at-a-time approach. Further, fermentation medium was optimized using Plackett-Burman (PB) and central composite designs (CCD). PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production. CCD results predicted the optimum concentrations of SS, CC, peptone, and NaCl as 7.8, 7.0, 6.5, and 2.7gL(-1), respectively to achieve maximum chitosanase activity (21.1Uml(-1)). Discovery of the novel optimal medium has improved chitosanase production by B. mojavensis up-to 9.5 folds. Lastly, 18.6Uml(-1) chitosanase activity was achieved in stirred tank bioreactor using optimal medium, which is quite satisfactory to proclaim this strain as a potential candidate to provide commercial chitosanase.en_US
dc.description.sponsorshipEge University Scientific Research Projects Coordination UnitEge University [15-FBE-11]en_US
dc.description.sponsorshipEge University Scientific Research Projects Coordination Unit, Grant number: 15-FBE-11en_US
dc.identifier.doi10.1002/jobm.201800132en_US
dc.identifier.endpage847en_US
dc.identifier.issn0233-111X
dc.identifier.issn1521-4028
dc.identifier.issue10en_US
dc.identifier.pmid30022499en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage836en_US
dc.identifier.urihttps://doi.org/10.1002/jobm.201800132
dc.identifier.urihttps://hdl.handle.net/11454/29932
dc.identifier.volume58en_US
dc.identifier.wosWOS:000446280400003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal of Basic Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBacillus mojavensisen_US
dc.subjectchitosanaseen_US
dc.subjectoptimizationen_US
dc.subjectstirred tank bioreactoren_US
dc.titleOptimization of chitosanase production by Bacillus mojavensis EGE-B-5.2ien_US
dc.typeArticleen_US

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