Biomolecular fingerprints of the effect of zoledronic acid on prostate cancer stem cells: Comparison of 2D and 3D cell culture models

Basit Öğe Kaydı
dc.authoridAcikgoz, Eda/0000-0002-6772-3081
dc.authoridMukhtarova, Gunel/0000-0003-3374-9272
dc.contributor.authorGuler, Gunnur
dc.contributor.authorAcikgoz, Eda
dc.contributor.authorMukhtarova, Guenel
dc.contributor.authorOktem, Gulperi
dc.date.accessioned2024-08-31T07:50:11Z
dc.date.available2024-08-31T07:50:11Z
dc.date.issued2024
dc.departmentEge Üniversitesien_US
dc.description.abstractRevealing the potential of candidate drugs against different cancer types without disrupting normal cells depends on the drug mode of action. In the current study, the drug response of prostate cancer stem cells (PCSCs) to zoledronic acid (ZOL) grown in two-dimensional (2D) and three-dimensional (3D) culture systems was compared using Fourier transform-infrared (FT-IR) spectroscopy which is a vibrational spectroscopic technique, supporting by biochemical assays and imaging techniques. Based on our data, in 2D cell culture conditions, the ZOL treatment of PCSCs isolated according to both C133 and CD44 cell surface properties induced early/late apoptosis and suppressed migration ability. The CD133 gene expression and protein levels were altered, depending on culture systems. CD133 expression was significantly reduced in 2D cells upon ZOL treatment. FT-IR data revealed that the integrity, fluidity, and ordering/disordering states of the cell membrane and nucleic acid content were altered in both 2D and 3D cells after ZOL treatment. Regular protein structures decrease in 2D cells while glycogen and protein contents increase in 3D cells, indicating a more pronounced cytotoxic effect of ZOL for 2D cells. Untreated 3D PCSCs exhibited an even different spectral profile associated with IR signals of lipids, proteins, nucleic acids, and glycogen in comparison to untreated 2D cells. Our study revealed significant differences in the drug response and cellular constituents between 2D and 3D cells. Exploring molecular targets and/or drug-action mechanisms is significant in cancer treatment approaches; thus, FT-IR spectroscopy can be successfully applied as a novel drug-screening method in clinical research.en_US
dc.identifier.doi10.1016/j.abb.2024.109920
dc.identifier.issn0003-9861
dc.identifier.issn1096-0384
dc.identifier.pmid38307315en_US
dc.identifier.scopus2-s2.0-85183994049en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.abb.2024.109920
dc.identifier.urihttps://hdl.handle.net/11454/105136
dc.identifier.volume753en_US
dc.identifier.wosWOS:001179769700001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofArchives of Biochemistry and Biophysicsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240831_Uen_US
dc.subjectProstate Cancer Stem Cellsen_US
dc.subjectFt-Ir Spectroscopyen_US
dc.subjectDrug -Action Mechanismen_US
dc.subjectMultivariate Statistical Analysisen_US
dc.subject3d Spheroidsen_US
dc.subject2d Monolayer Cellsen_US
dc.subjectApoptosisen_US
dc.subjectMigrationen_US
dc.subjectCd133 Expressionen_US
dc.titleBiomolecular fingerprints of the effect of zoledronic acid on prostate cancer stem cells: Comparison of 2D and 3D cell culture modelsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu