A microbial biosensor based on bacterial cells immobilized on chitosan matrix
Küçük Resim Yok
Tarih
2009
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Sa
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A bio-electrochemical system consisting of Gluconobacter oxydans DSM 2343 cells as a biological material and carbon nanotube (CNT)-free and CNT-modified chitosan as immobilizing matrices has been developed. The measurement was based on the respiratory activity of the cells estimated by the oxygen consumption at -0.7 V (versus the Ag/AgCl reference electrode) due to the metabolic activity in the presence of substrates. The system was calibrated and dependence of signal amplitude on the measuring conditions and cell amount was studied as well as the substrate specificity, pH, temperature and working potential. The biosensors (CNT-modified and unmodified) were demonstrated for the quantification of glucose in the range of 0.05-1.0 mM, at 30 degrees C and pH 7.0 with the 40 s of response time. The linear relationships between sensor response (y; mu A/cm(2)) and substrate concentration (x; mM) were defined by the equations of y=1.160x+0.151 (R-2=0.990) and y = 1.261x+0.197 (R-2 = 0.982), respectively. All other data were also given as comparison of two systems one with CNT-modified and CNT-free. (c) 2009 Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
Microbial biosensor, Chitosan, Carbon nanotube, Gluconobacter oxydans, Electrochemical biosensor
Kaynak
Bioelectrochemistry
WoS Q Değeri
Q1
Scopus Q Değeri
Q2
Cilt
75
Sayı
1
