Offline Glucose Biomonitoring in Yeast Culture by Polyamidoamine/Cysteamine-Modified Gold Electrodes

dc.contributor.authorYuksel, Merve
dc.contributor.authorAkin, Mehriban
dc.contributor.authorGeyik, Caner
dc.contributor.authorDemirkol, Dilek Odaci
dc.contributor.authorOzdemir, Caglar
dc.contributor.authorBluma, Arne
dc.contributor.authorHoepfner, Tim
dc.contributor.authorBeutel, Sascha
dc.contributor.authorTimur, Suna
dc.contributor.authorScheper, Thomas
dc.date.accessioned2019-10-27T21:24:55Z
dc.date.available2019-10-27T21:24:55Z
dc.date.issued2011
dc.departmentEge Üniversitesien_US
dc.description.abstractThis article deals with the use of pyranose oxidase (PyOx) and glucose oxidase (GOx) enzymes in amperometric biosensor design and their application in monitoring fermentation processes with the combination of flow injection analysis (FIA). The amperometric studies were carried out at -0.7 V by following the oxygen consumption due to the enzymatic reactions for both batch and FIA modes. Optimization studies (enzyme amounts and pH) and analytical parameters such as linearity, repeatability, effect of interference, storage, and operational stabilities have been studied. Under optimized conditions, for the PyOx-based biosensor, linear graph was obtained from 0.025 to 0.5 mM glucose in phosphate buffer ( 50 mM) at pH 7.0 with the equation of y = 3.358x + 0.028 and R-2 = 0.998. Linearity was found to be 0.01-1.0 mM in citrate buffer (50 mM and pH 4.0) with the equation of y = 1.539x + 0.181 and R-2 = 0.992 for the GOx biosensor. Finally, these biosensor configurations were further evaluated in a conventional flow injection system. Results from batch experiments provide a guide to design sensitive, stable, and interference-free biosensors for FIA mode. Biosensor stability, dynamic range, and repeatability were also studied in FIA conditions, and the applicability for the determination of glucose in fermentation medium could be successfully demonstrated. The FIA-combined glucose biosensor was used for the offline monitoring of yeast fermentation. The obtained results correlated well with HPLC measurements. (C) 2011 American Institute of Chemical Engineers Biotechnol. Prog., 27: 530-538, 2011en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK); Federal Ministry of Education and Research (BMBF)Federal Ministry of Education & Research (BMBF) [108T293]; EBILTEM (Ege University, Research and Application Center of Science and Technology)Ege University; Ege University Research FoundationEge University [2009 Fen 0059]en_US
dc.description.sponsorshipThis work was supported by Scientific and Technological Research Council of Turkey (TUBITAK) and Federal Ministry of Education and Research (BMBF) under the Research Project number 108T293. EBILTEM (Ege University, Research and Application Center of Science and Technology) and Ege University Research Foundation (Project number 2009 Fen 0059) were also acknowledged for the research grants.en_US
dc.identifier.doi10.1002/btpr.544en_US
dc.identifier.endpage538en_US
dc.identifier.issn8756-7938
dc.identifier.issn1520-6033
dc.identifier.issue2en_US
dc.identifier.pmid21485034en_US
dc.identifier.startpage530en_US
dc.identifier.urihttps://doi.org/10.1002/btpr.544
dc.identifier.urihttps://hdl.handle.net/11454/44699
dc.identifier.volume27en_US
dc.identifier.wosWOS:000289431600028en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWiley-Blackwellen_US
dc.relation.ispartofBiotechnology Progressen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectflow injection analysisen_US
dc.subjectglucose biomonitoringen_US
dc.subjectglucose oxidaseen_US
dc.subjectpyranose oxidaseen_US
dc.subjectself-assembled monolayersen_US
dc.titleOffline Glucose Biomonitoring in Yeast Culture by Polyamidoamine/Cysteamine-Modified Gold Electrodesen_US
dc.typeArticleen_US

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