The Risk Factors and Clinical Features of Acanthamoeba Keratitis: First Time Detection of Acanthamoeba T5 Genotype from Keratitis Patients in Turkey

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dc.authoridGunduz, Cumhur/0000-0002-6593-3237
dc.authoridDirim Erdogan, Derya/0000-0001-6927-9917
dc.authorscopusid35795253800
dc.authorscopusid55334665700
dc.authorscopusid57202756148
dc.authorscopusid23989747000
dc.authorscopusid24077472700
dc.authorscopusid56638649300
dc.authorscopusid7003943383
dc.authorwosidDelibaş, Songül Bayram/ADB-3199-2022
dc.authorwosidGunduz, Cumhur/GOP-0629-2022
dc.contributor.authorErdogann, Derya Dirim
dc.contributor.authorAykur, Mehmet
dc.contributor.authorGunel, Nur Selvi
dc.contributor.authorPalamar, Melis
dc.contributor.authorSelver, Ozlem Barut
dc.contributor.authorOzel, Buket
dc.contributor.authorYagci, Ayse
dc.date.accessioned2023-01-12T19:51:02Z
dc.date.available2023-01-12T19:51:02Z
dc.date.issued2022
dc.departmentN/A/Departmenten_US
dc.description.abstractPurpose The primary aim of this study is to investigate Acanthamoeba in clinical samples of keratitis cases (n = 60), in contact lens (CL) and lens care solutions of asymptomatic CL users (n = 41), and to identify the genotypes in positive samples. The secondary aim is to assess the risk factors and clinical features of Acanthamoeba keratitis (AK) patients. Methods All samples from patients and asymptomatic CL users were examined by microscopy and inoculated in non-nutrient agar plates. PCR was performed using the DNA isolated from corneal scrapings, CL and lens care solution samples. Positive DNA samples were sequenced to determine the genotype of Acanthamoeba. Results In none of the samples, Acanthamoeba was identified by microscopy, while Acanthamoeba was detected in a patient with keratitis by culture method. However, Acanthamoeba was detected in 11.66% (7/60) of the keratitis patients by PCR. The genotypes of these isolates detected by sequencing were T4 (4), and T5 (3). Acanthamoeba was detected in none of the samples of asymptomatic CL users by any of the three methods. Conclusion To best of our knowledge, this is the first study to detect T5 genotype in AK patients from Turkey. In addition, the CL use was found to be an important risk factor for AK.en_US
dc.description.sponsorshipScientific Research Projects Branch Directorate of Ege University, Turkey [2011-TIP-078]en_US
dc.description.sponsorshipThis study was funded by Scientific Research Projects Branch Directorate of Ege University, Turkey (Grant Number: 2011-TIP-078).en_US
dc.identifier.doi10.1007/s11686-022-00596-4
dc.identifier.endpage1392en_US
dc.identifier.issn1230-2821
dc.identifier.issn1896-1851
dc.identifier.issue3en_US
dc.identifier.pmid35864410en_US
dc.identifier.scopus2-s2.0-85134533345en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1384en_US
dc.identifier.urihttps://doi.org/10.1007/s11686-022-00596-4
dc.identifier.urihttps://hdl.handle.net/11454/76210
dc.identifier.volume67en_US
dc.identifier.wosWOS:000828425600001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer Int Publ Agen_US
dc.relation.ispartofActa Parasitologicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAcanthamoebaen_US
dc.subjectKeratitisen_US
dc.subjectCorneaen_US
dc.subjectContact lensen_US
dc.subjectGenotypeen_US
dc.subjectT5en_US
dc.subjectIdentificationen_US
dc.subjectDiagnosisen_US
dc.subjectT4en_US
dc.subjectSamplesen_US
dc.subjectWateren_US
dc.subjectSpp.en_US
dc.titleThe Risk Factors and Clinical Features of Acanthamoeba Keratitis: First Time Detection of Acanthamoeba T5 Genotype from Keratitis Patients in Turkeyen_US
dc.typeArticleen_US

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