A novel Lactiplantibacillus plantarum strain: probiotic properties and optimization of the growth conditions by response surface methodology

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dc.authoridGÖKMEN, Gökhan Gurur/0000-0002-1180-0682
dc.authoridDÜZEL, Ahmet/0000-0003-4106-4548
dc.authoridKIŞLA, Duygu/0000-0002-6993-3883
dc.authoridGoksungur, Yekta/0000-0002-5166-6561
dc.authoridAslan, Emek/0000-0002-1287-3322
dc.authoridSARGIN, SAIT/0000-0002-5037-3193
dc.contributor.authorGokmen, Goekhan Gurur
dc.contributor.authorSariyildiz, Seda
dc.contributor.authorCholakov, Remzi
dc.contributor.authorNalbantsoy, Ayse
dc.contributor.authorBaler, Biray
dc.contributor.authorAslan, Emek
dc.contributor.authorDuzel, Ahmet
dc.date.accessioned2024-08-31T07:50:11Z
dc.date.available2024-08-31T07:50:11Z
dc.date.issued2024
dc.departmentEge Üniversitesien_US
dc.description.abstractThe objective of this study is to explore the probiotic properties and optimal growth conditions of Lactiplantibacillus plantarum BG24. L. plantarum BG24 exhibited a remarkable ability to utilize lactose, and to grow under acidic conditions and in the presence of high levels of bile salts. The strain showed the highest antibacterial activity against L. monocytogenes Scott A (zone of inhibition: 26 mm). L. plantarum BG24 was found to be resistant to 8 of the tested 19 antibiotics using the disc diffusion method.and its multiple antibiotic resistance (MAR) index was calculated as 0.421. The adhesion rate to human intestinal epithelial Caco-2 cells was determined as 37.51%. The enzyme profile of L. plantarum BG24 was investigated using API ZYM test kit and the highest enzymatic activities were found for Leucine arylamidase, beta-glucosidase, Valine arylamidase, beta-galactosidase and N-acetyl-beta-glucosaminidase. L. plantarum BG24 strain showed higher microbial growth under static conditions (6.60 OD600) compared to 100 rpm (5.73 OD600) and 200 rpm (5.02 OD600) shaking speed due to its facultative anaerobic characteristic. However, different inoculation rates and glucose addition did not make a statistically significant difference on biomass formation (p > 0.05). The specific growth rate of L. plantarum BG24 was 0.416 h(-1), the doubling time was 1.67 h, and the biomass productivity value was 0.14 gL(-1) h(-1) in the original MRS broth (pH 5.7) while higher values were found as 0.483 h(-1), 1.43 h and 0.17 gL(-1) h(-1), respectively, in MRS broth (pH 6.5) medium enriched with 5 g/L yeast extract. The stirred tank bioreactor was used to optimise the growth of BG24 strain. The process variables was optimized at 0.05 vvm of aeration rate, 479 rpm of agitation speed, 3% of inoculation rate and 18 h of incubation time. The maximum biomass (g/L) production was obtained as 3.84 g/L at the optimized conditions.en_US
dc.description.sponsorshipEge University Research Foundationen_US
dc.description.sponsorshipNo Statement Availableen_US
dc.identifier.doi10.1007/s11274-023-03862-3
dc.identifier.issn0959-3993
dc.identifier.issn1573-0972
dc.identifier.issue2en_US
dc.identifier.pmid38194015en_US
dc.identifier.scopus2-s2.0-85181720038en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1007/s11274-023-03862-3
dc.identifier.urihttps://hdl.handle.net/11454/105133
dc.identifier.volume40en_US
dc.identifier.wosWOS:001138441700001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofWorld Journal of Microbiology & Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240831_Uen_US
dc.subjectProbioticen_US
dc.subjectLactiplantibacillus Plantarumen_US
dc.subjectBiomass Productionen_US
dc.subjectResponse Surface Methodologyen_US
dc.titleA novel Lactiplantibacillus plantarum strain: probiotic properties and optimization of the growth conditions by response surface methodologyen_US
dc.typeArticleen_US

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