Yeni nesil biyomalzemelerin geliştirilmesi ve medikal alandaki kullanım olanaklarının araştırılması
Yükleniyor...
Tarih
2018
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Ege Üniversitesi, Fen Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Hızla gelişmekte olan doku mühendisliği üzerine çalışmalar giderek artmaktadır. Geleceğin dışarda geliştirilecek organlarının ilk basamağını oluşturan doku iskeleleri ve üç boyutlu hücre kültürü kavramları biyomalzemelerin bu alandaki önemine dikkat çekmektedir. Bu alanda kullanılmak üzere moleküler afinite yaklaşımıyla geliştirilen biyomalzemelerden biri de moleküler baskılanmış polimer teknolojisidir. Sunulan bu doktora tezi kapsamında moleküler baskılanmış nanopolimerlerin geliştirilip, biyoparçalanabilir proteinik kriyojel sistemlerine gömülmesiyle elde edilen hibrit afinite sistemleri, üç boyutlu hücre kültürü ve doku mühendisliği uygulamalarında kullanılmak üzere önerilmiştir. Bu kapsamda hücre yüzeyinde bulunan sialic asit (NANA) molekülleri kalıp olarak kullanılmış ve üç farklı NANA baskılanmış nanopolimer geliştirilmiştir. Kontrol olarak aynı kimyasal özelliğe sahip baskılanmamış nanopolimerler de hazırlanmıştır. Geliştirilen bu nanopolimerlere sialik asit adsorpsiyonu farklı koşullarda incelenmiş ve bağlanma karakteristikleri belirlenmiştir. Bunun yanında kriyojelasyon ile makroporöz yapıda albümin iskeleleri hazırlanmış ve karakterize edilmiştir. Geliştirilen nanopolimerler albümin kriyojelleriyle birleştirilerek nanopolimer gömülü kriyojel sistemleri hazırlanmış ve karakterize edilmiştir. Tezin son aşamasında üç farklı tipteki hücre ile üç boyutlu hücre kültürü çalışmaları yapılmıştır. Sonuç olarak çeşitli biyomedikal uygulamalarda kullanılabilecek yeni nesil hibrit biyomalzemeler elde edilmiştir.
Studies on rapidly developing tissue engineering field are increasing. Tissue scaffolds and three-dimensional cell culture concepts, which constitute the first step of the organs to be developed outside of the future, draw attention to the importance of biomaterials in this area. One of the biomaterials developed with the molecular affinity approach for use in this field is molecularly imprinted polymer technology. In this doctoral thesis, hybrid affinity systems obtained by developing and imprinting molecularly imprinted nanopolymers into biodegradable proteinaceous cryogel systems have been proposed for use in three-dimensional cell culture and tissue engineering applications. In this context, sialic acid (NANA) molecules on the cell surface were used as template and three different NANA-imprinted nanopolymers were developed. Developed nanopolymers with the same chemical properties were also prepared as the control. The sialic acid adsorption on these developed nanopolymers was investigated under different conditions and their binding characteristics were determined. In addition, macroporous albumin scaffolds were prepared and characterized by cryogelation process. Nanopolymer embedded cryogel systems were prepared and characterized by combining the developed nanopolymers with albumin cryogels. Three-dimensional cell culture studies with three different types of cells were carried out at the end of the thesis. As a result, new generation hybrid biomaterials have been obtained which can be used in various biomedical applications.
Studies on rapidly developing tissue engineering field are increasing. Tissue scaffolds and three-dimensional cell culture concepts, which constitute the first step of the organs to be developed outside of the future, draw attention to the importance of biomaterials in this area. One of the biomaterials developed with the molecular affinity approach for use in this field is molecularly imprinted polymer technology. In this doctoral thesis, hybrid affinity systems obtained by developing and imprinting molecularly imprinted nanopolymers into biodegradable proteinaceous cryogel systems have been proposed for use in three-dimensional cell culture and tissue engineering applications. In this context, sialic acid (NANA) molecules on the cell surface were used as template and three different NANA-imprinted nanopolymers were developed. Developed nanopolymers with the same chemical properties were also prepared as the control. The sialic acid adsorption on these developed nanopolymers was investigated under different conditions and their binding characteristics were determined. In addition, macroporous albumin scaffolds were prepared and characterized by cryogelation process. Nanopolymer embedded cryogel systems were prepared and characterized by combining the developed nanopolymers with albumin cryogels. Three-dimensional cell culture studies with three different types of cells were carried out at the end of the thesis. As a result, new generation hybrid biomaterials have been obtained which can be used in various biomedical applications.
Açıklama
Anahtar Kelimeler
Albümin Kriyojel, Moleküler Baskılanmış Nanopolimerler, Sialik Asit (NANA), Üç Boyutlu Hücre Kültürü, Doku Mühendisliği, Albumin Cryogel, Molecularly İmprinted Nanopolymers, Sialic Acid (NANA), Three Dimensional Cell Culture, Tissue Engineering
