Whole cell immobilized amperometric biosensor based on Saccharomyces cerevisiae for selective determination of vitamin B-1 (thiamine)

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dc.contributor.authorAkyilmaz, E
dc.contributor.authorYasa, I
dc.contributor.authorDinckaya, E
dc.date.accessioned2019-10-27T19:17:48Z
dc.date.available2019-10-27T19:17:48Z
dc.date.issued2006
dc.departmentEge Üniversitesien_US
dc.description.abstractA new amperometric whole cell biosensor based on Saccharomyces cerevisiae immobilized in gelatin was developed for selective determination of vitamin B, (thiamine). The biosensor was constructed by using gelatin and crosslinking agent glutaraldehyde to immobilize S. cerevisiae cells on the Teflon membrane of dissolved oxygen (DO) probe used as the basic electrode system combined with a digital oxygen meter. The cells were induced by vitamin 131 in the culture medium, and the cells used it as a carbon source in the absence of glucose. So, when the vitamin 131 solution is injected into the whole cell biosensor system, an increase in respiration activity of the cells results from the metabolic activity and causes a decrease in the DO concentration of interval surface of DO probe related to vitamin B, concentration. The response time of the biosensor is 3 min, and the optimal working conditions of the biosensor were carried out as pH 7.0, 50mM Tris-HCl, and 30 degrees C. A linear relationship was obtained between the DO concentration decrease and vitamin B, concentration between 5.0 x 10(-3) and 10(-1) mu M. In the application studies of the biosensor, sensitive determination of vitamin 131 in the vitamin tablets was investigated. (c) 2006 Elsevier Inc. All rights reserved.en_US
dc.identifier.doi10.1016/j.ab.2006.04.019en_US
dc.identifier.endpage84en_US
dc.identifier.issn0003-2697
dc.identifier.issue1en_US
dc.identifier.pmid16701070en_US
dc.identifier.startpage78en_US
dc.identifier.urihttps://doi.org/10.1016/j.ab.2006.04.019
dc.identifier.urihttps://hdl.handle.net/11454/38478
dc.identifier.volume354en_US
dc.identifier.wosWOS:000238394600011en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.relation.ispartofAnalytical Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectS. cerevisiaeen_US
dc.subjectwhole cell biosensoren_US
dc.subjectvitamin B-1en_US
dc.subjectthiamineen_US
dc.subjectmicrobial biosensoren_US
dc.subjectbiosensoren_US
dc.titleWhole cell immobilized amperometric biosensor based on Saccharomyces cerevisiae for selective determination of vitamin B-1 (thiamine)en_US
dc.typeArticleen_US

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