Surface Modification with a Catechol-Bearing Polypeptide and Sensing Applications

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dc.contributor.authorAydindogan, Eda
dc.contributor.authorCelik, Emine Guler
dc.contributor.authorDemirkol, Dilek Odaci
dc.contributor.authorYamada, Shuhei
dc.contributor.authorEndo, Takeshi
dc.contributor.authorTimur, Suna
dc.contributor.authorYagci, Yusuf
dc.date.accessioned2019-10-27T10:04:33Z
dc.date.available2019-10-27T10:04:33Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractA novel catechol-bearing polypeptide (CtP) was synthesized and used as a component of electrochemical biosensor involving both enzymatic activity and affinity-based sensing systems. Glucose oxidase (GOx) and anti-immunoglobulin G (Anti-IgG) were selected as model biorecognition elements for the selective analysis of glucose and IgG. Step-by-step surface modifications were followed using various techniques such as cyclic voltammetry (CV) and electrochemical impedance spectrometry (EIS) as well as X-ray photoelectron spectroscopy (XPS). Additionally, contact angles were measured in order to observe surface properties. Amperometric measurements using the GOx biosensor were performed at -0.7 V by following the oxygen consumption due to the enzymatic reaction in different glucose concentrations. Affinity-based interactions via IgG sensor were monitored using the differential pulse voltammetry (DPV) technique. As the "surface design with CtP" approach employed herein is generally applicable and easily adaptable to obtain functional matrices for biomolecule immobilization, CtP-coated surfaces can be promising platforms for the fabrication of various biobased sensing systems.en_US
dc.description.sponsorshipIstanbul Technical University Foundation; Ege University Research FoundationEge Universityen_US
dc.description.sponsorshipIstanbul Technical University and Ege University Research Foundations are acknowledged for the financial support.en_US
dc.identifier.doi10.1021/acs.biomac.8b00650en_US
dc.identifier.endpage3076en_US
dc.identifier.issn1525-7797
dc.identifier.issn1526-4602
dc.identifier.issue7en_US
dc.identifier.pmid29750865en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage3067en_US
dc.identifier.urihttps://doi.org/10.1021/acs.biomac.8b00650
dc.identifier.urihttps://hdl.handle.net/11454/30253
dc.identifier.volume19en_US
dc.identifier.wosWOS:000438470800070en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmer Chemical Socen_US
dc.relation.ispartofBiomacromoleculesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleSurface Modification with a Catechol-Bearing Polypeptide and Sensing Applicationsen_US
dc.typeArticleen_US

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