Production of sophorolipids from whey II. Product composition, surface active properties, cytotoxicity and stability against hydrolases by enzymatic treatment

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dc.contributor.authorOtto, RT
dc.contributor.authorDaniel, HJ
dc.contributor.authorPekin, G
dc.contributor.authorMuller-Decker, K
dc.contributor.authorFurstenberger, G
dc.contributor.authorReuss, M
dc.contributor.authorSyldatk, C
dc.date.accessioned2019-10-27T18:24:53Z
dc.date.available2019-10-27T18:24:53Z
dc.date.issued1999
dc.departmentEge Üniversitesien_US
dc.description.abstractSophorolipids. obtained by a two-stage process starting from deproteinized whey concentrate using Cryptococcus curvatus ATCC 20509 and Candida bombicola ATCC 22214, were compared to products from one-stage processes, using different lipidic compounds as substrates. Results showed that above all carbon source and not cultivation conditions had a distinct influence on the composition of the crude product mixture and therefore on the physicochemical and biological properties of the sophorolipids, such as, for example, surface activity, cytotoxicity and stability against hydrolases. The results were completed by corresponding data for purified mono- and diacetylated (17-hydroxyoctadecenoic)-1',4 "-lactonized sophorolipids. Crude sophorolipid mixtures showed moderate to good surface active properties (SFTmin 39 mN m(-1), CMC 130 mg l(-1)), water solubilities (2-3 g l(-1)) and low cytotoxicities (LC50 300-700 mg l(-1)). In contrast, purified sophorolipids were more surface active (SFTmin 36 mN m(-1), CMC 10 mg l(-1)), less water soluble (max. 70 mg l(-1)) and showed stronger cytotoxic effects (LC50 15 mg l(-1)). Incubation of crude sophorolipid mixtures with different hydrolases demonstrated that treatment with commercially available lipases such as from Candida rugosa and Mucor miehei distinctly reduced the surface active properties of the sophorolipids, while treatment with porcine liver esterase and glycosidases had no effect.en_US
dc.identifier.doi10.1007/s002530051551en_US
dc.identifier.endpage501en_US
dc.identifier.issn0175-7598
dc.identifier.issue4en_US
dc.identifier.pmid10570796en_US
dc.identifier.startpage495en_US
dc.identifier.urihttps://doi.org/10.1007/s002530051551
dc.identifier.urihttps://hdl.handle.net/11454/36246
dc.identifier.volume52en_US
dc.identifier.wosWOS:000083529900006en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer Verlagen_US
dc.relation.ispartofApplied Microbiology and Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleProduction of sophorolipids from whey II. Product composition, surface active properties, cytotoxicity and stability against hydrolases by enzymatic treatmenten_US
dc.typeArticleen_US

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