Preparation and properties of ?-galactosidase chemically attached to activated chitin

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dc.contributor.authorÖnal S.
dc.contributor.authorTelefoncu A.
dc.date.accessioned2019-10-27T08:59:38Z
dc.date.available2019-10-27T08:59:38Z
dc.date.issued2003
dc.departmentEge Üniversitesien_US
dc.description.abstract?-Galactosidase (?-D-galactoside galactohydrolase, EC 3.2.1.22) from watermelon was covalently immobilized on chitin. The immobilized ?-galactosidase exhibited an activity of 0.61 U per g of carrier and an activity yield of 67%. The properties of free and immobilized ?-galactosidase were also searched and compared. The results showed that, optimum conditions for activity were not affected by immobilization. The optimum pH and temperature for free and immobilized enzyme found as pH 6.0 and 65°C, respectively. Compared with the free enzyme, the temperature and pH stabilities of the immobilized enzyme were similar. Both the enzymes were stable between pH 2-10 and below 50°C. The Km values for free and immobilized enzyme were determined using p-nitrophenyl-?-D-galactopyranoside (PNPG) and raffinose as substrates. Operational stability of the immobilized enzyme was investigated by using both substrates. The operational half-life (t 1/2) was calculated as 34 h for PNPG and 28 h for raffinose. The immobilized ?-galactosidase was also utilized in the hydrolysis of raffinose. The immobilization procedure on chitin was cheap and also easy to carry out, and the immobilized enzyme had good properties that the potential for practical application is considerable.en_US
dc.description.sponsorshipFEN 95/025en_US
dc.description.sponsorshipThe authors are grateful for the financial support provided by the Ege University Research Foundation under project FEN 95/025. --en_US
dc.identifier.doi10.1081/BIO-120023163en_US
dc.identifier.endpage355en_US
dc.identifier.issn1073-1199
dc.identifier.issue3en_US
dc.identifier.pmid12906314en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage339en_US
dc.identifier.urihttps://doi.org/10.1081/BIO-120023163
dc.identifier.urihttps://hdl.handle.net/11454/27868
dc.identifier.volume31en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofArtificial Cells, Blood Substitutes, and Immobilization Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject?-Galactosidaseen_US
dc.subject1,1'-Carbonyldiimidazoleen_US
dc.subjectChitinen_US
dc.subjectCovalent attachmenten_US
dc.subjectImmobilizationen_US
dc.subjectRaffinoseen_US
dc.subjectWatermelonen_US
dc.titlePreparation and properties of ?-galactosidase chemically attached to activated chitinen_US
dc.typeArticleen_US

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