Investigation of in vitro antileishmanial activity of moxifloxacin, linezolid and caspofungin on leishmania tropica promastigotes
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Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmada, Leishmania tropica üzerine moksifloksasin ve linezolid ile kaspofunginin, potansiyel anti-leishmanial etkilerinin in vitro olarak araştırılması amaçlandı. Yöntemler: Tüm ajanların in vitro etkisi mikrodilüsyon yöntemiyle araştırıldı. Bu amaçla moksifloksasin, linezolid ve kaspofunginin 4096 ?g/mL-0.008 ?g/mL arasındaki konsantrasyonlarda seri dilüsyonları yapıldı. Ardından uygun besiyerinde inkübe edilen promastigotlar hemositometre ile sayıldı ve besiyeri+antibiyotik veya antifungal içeren kuyucuklardaki son konsantrasyonları 2.5x106 hücre/mL olacak şekilde ayarlandı. İnkübasyondan sonra canlı promastigotlar hemositometre ile sayıldı ve ajanların %50 inhibitör konsantrasyonları (IK50) kontrollerle karşılaştırılarak belirlendi. Bulgular: Moksifloksasin, linezolid ve kaspofunginin in vitro IK50 değerleri sırasıyla 194.7 ?g/mL, 896 ?g/mL ve 235.7 ?g/mL olarak bulundu. Sonuç: Moksifloksasinin, L. tropica promastigotlarına karşı çalışılan diğer ajanlara göre daha düşük konsantrasyonlarda etkili olduğu sonucuna varıldı. (Turkiye Parazitol Derg 2013; 37: 1-3)
Objective: This study aimed to evaluate the potential in vitro anti-leishmanial activities of moxifloxacin, linezolid and caspofungin against Leishmania tropica. Methods: In vitro effects of all agents were studied by using the microdilution method. For this purpose, serial dilutions of the aforementioned agents were prepared in concentrations between 4096 μg/mL-0.008 μg/mL. Afterwards, promastigotes incubated in suitable medium were counted with the hemocytometer and adjusted as having a last concentration of 2.5x106 cells/mL in wells containing medium+antibiotic or antifungal. After incubation live promastigotes were counted with the hemocytometer and inhibitor concentrations (IC50) were determined by comparing with the control that contained no antibiotics or antifungal. Results: IC50 values of moxiloxacin, linezolid and caspofungin were found as 194.7 μg/mL, 896 μg/mL and 235.7 μg/mL, respectively. Conclusion: As a result, moxifloxacin was found to be effective in lower concentrations than the other studied agents against L. tropica promastigotes. (Turkiye Parazitol Derg 2013; 37: 1-3)
Objective: This study aimed to evaluate the potential in vitro anti-leishmanial activities of moxifloxacin, linezolid and caspofungin against Leishmania tropica. Methods: In vitro effects of all agents were studied by using the microdilution method. For this purpose, serial dilutions of the aforementioned agents were prepared in concentrations between 4096 μg/mL-0.008 μg/mL. Afterwards, promastigotes incubated in suitable medium were counted with the hemocytometer and adjusted as having a last concentration of 2.5x106 cells/mL in wells containing medium+antibiotic or antifungal. After incubation live promastigotes were counted with the hemocytometer and inhibitor concentrations (IC50) were determined by comparing with the control that contained no antibiotics or antifungal. Results: IC50 values of moxiloxacin, linezolid and caspofungin were found as 194.7 μg/mL, 896 μg/mL and 235.7 μg/mL, respectively. Conclusion: As a result, moxifloxacin was found to be effective in lower concentrations than the other studied agents against L. tropica promastigotes. (Turkiye Parazitol Derg 2013; 37: 1-3)
Açıklama
Anahtar Kelimeler
Parazitoloji, Genel ve Dahili Tıp
Kaynak
Türkiye Parazitoloji Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
37
Sayı
1