Promoter hypermethylation-mediated down-regulation of RUNX3 gene in human brain tumors

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dc.contributor.authorAvci, C. B.
dc.contributor.authorDodurga, Y.
dc.contributor.authorSusluer, S. Y.
dc.contributor.authorSigva, Z. O. D.
dc.contributor.authorYucebas, M.
dc.contributor.authorCaglar, H. O.
dc.contributor.authorAkalin, T.
dc.contributor.authorDalbasti, T.
dc.contributor.authorOktar, N.
dc.contributor.authorGunduz, C.
dc.date.accessioned2019-10-27T22:14:34Z
dc.date.available2019-10-27T22:14:34Z
dc.date.issued2014
dc.departmentEge Üniversitesien_US
dc.description.abstractBackground The Runx family proteins, including RUNX3, are tissue-restricted transcription factors and play role in neuronal development and tumorigenesis. RUNX3 has an important role in glioblastoma (GBM) tumorigenesis because of its promoter hypermethylation. Aim We aimed to evaluate the methylation-mediated expression regulation of RUNX3 gene in brain tumors. Patients and methods Cases of meningiomas WHO grade III (3), anaplastic astrocytomas (3), diffuse astrocytoma (3), and GBM (12) were recruited into this study. Real-time quantitative PCR was performed for analyses of DNA promoter methylation and analyses of methylation-mediated expression status of RUNX3 gene was performed by real-time quantitative RT-PCR. Results There was no significant difference between methylated and unmethylated quantitative ratio of RUNX3 gene promoter region and also no significant difference in relative ratio of RUNX3 gene expression in brain tumor groups. Methylated and unmethylated ratio in anaplastic astrocytoma, diffuse astrocytoma, GBM, meningioma (WHO grade III) and in all groups were; 1.44, 1.09, 1.51, 1.52 and 1.43, respectively. One allele was found methylated necessarily. No methylation was detected in one case of GBM group and one case of anaplastic astrocytoma group. There was no unmethylated promoter in one of the GBM cases. There were significant differences between relative ratio of RUNX3 gene expression and methylated/unmethylated ratio rates for all cases (p = 0.001) and GBM groups (p = 0.041). Conclusion This study overemphasized the RUNX3 gene importance in brain tumors, due to the existence of at least one methylated allele.en_US
dc.description.sponsorshipEge University Medical Faculty Research Project SubcommitteeEge University [T-00033 APAK]en_US
dc.description.sponsorshipThis study is supported by Ege University Medical Faculty Research Project Subcommittee (Grant number: T-00033 APAK).en_US
dc.identifier.doi10.1007/s11845-013-1001-3en_US
dc.identifier.endpage264en_US
dc.identifier.issn0021-1265
dc.identifier.issn1863-4362
dc.identifier.issue2en_US
dc.identifier.pmid23934435en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage259en_US
dc.identifier.urihttps://doi.org/10.1007/s11845-013-1001-3
dc.identifier.urihttps://hdl.handle.net/11454/50032
dc.identifier.volume183en_US
dc.identifier.wosWOS:000339819100015en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer London Ltden_US
dc.relation.ispartofIrish Journal of Medical Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHuman brain tumorsen_US
dc.subjectRUNX3en_US
dc.subjectDNA methylationen_US
dc.subjectGene expressionen_US
dc.titlePromoter hypermethylation-mediated down-regulation of RUNX3 gene in human brain tumorsen_US
dc.typeArticleen_US

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