Immunoinformatics Approach to Design a Multi-Epitope Vaccine against Cutaneous Leishmaniasis

Basit Öğe Kaydı
dc.authoridNaz, Shumaila/0000-0001-8088-0084
dc.authorscopusid57225695870
dc.authorscopusid57223381402
dc.authorscopusid23767207200
dc.authorscopusid57189602696
dc.authorscopusid55665073900
dc.authorscopusid6603630554
dc.authorscopusid51863115700
dc.contributor.authorNaz, Shumaila
dc.contributor.authorAroosh, Aiman
dc.contributor.authorCaner, Ayse
dc.contributor.authorSahar, Esra Atalay
dc.contributor.authorToz, Seray
dc.contributor.authorOzbel, Yusuf
dc.contributor.authorAbbasi, Sumra Wajid
dc.date.accessioned2024-08-25T18:53:18Z
dc.date.available2024-08-25T18:53:18Z
dc.date.issued2023
dc.departmentEge Üniversitesien_US
dc.description.abstractCutaneous Leishmaniasis (CL), a neglected vector-borne disease caused by protozoan parasite Leishmania major (L. major), is a major public health concern, and the development of new strategies to reduce the disease incidence has become a top priority. Advances in immunoinformatics and in-silico epitope prediction could be a promising approach to designing a finest vaccine candidate. In this study, we aimed to design a peptide-based vaccine against CL using computational tools and identified ten B-cell-derived T-cell epitopes from the glycoprotein gp63 of L. major. All of the potential immunodominant epitopes were used to design a vaccine construct along with a linker and an adjuvant at the N-terminal for enhancing its immunogenicity. Additionally, many characteristics of the proposed vaccine were examined, and it was confirmed to be non-allergenic, non-toxic, and thermally stable. To assess the vaccine interaction with the innate immune toll-like receptor-4 (TLR-4), a 3D structure of the vaccine construct was developed. Molecular docking and molecular dynamic simulation were used to confirm the binding and to assess the stability of the vaccine-TLR4 complex and interactions, respectively. In conclusion, our multi-epitope vaccine will provide a gateway to analyze the protein function of a potential vaccine candidate against CL.en_US
dc.identifier.doi10.3390/vaccines11020339
dc.identifier.issn2076-393X
dc.identifier.issue2en_US
dc.identifier.pmid36851219en_US
dc.identifier.scopus2-s2.0-85149149615en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.3390/vaccines11020339
dc.identifier.urihttps://hdl.handle.net/11454/103012
dc.identifier.volume11en_US
dc.identifier.wosWOS:000941215300001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofVaccinesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240825_Gen_US
dc.subjectLeishmania majoren_US
dc.subjectcutaneous leishmaniasisen_US
dc.subjectglycoproteinen_US
dc.subjecttoll-like receptor-4en_US
dc.subjectmolecular dynamic simulationen_US
dc.subjectProtein-Structureen_US
dc.subjectProtectionen_US
dc.subjectPeptidesen_US
dc.subjectRefinementen_US
dc.subjectPredictionen_US
dc.subjectDiagnosisen_US
dc.subjectAntigensen_US
dc.subjectCellsen_US
dc.subjectModelen_US
dc.titleImmunoinformatics Approach to Design a Multi-Epitope Vaccine against Cutaneous Leishmaniasisen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu