Secondary metabolites from Astragalus karjaginii BORISS and the evaluation of their effects on cytokine release and hemolysis

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dc.contributor.authorAslanipour, Behnaz
dc.contributor.authorGulcemal, Derya
dc.contributor.authorNalbantsoy, Ayse
dc.contributor.authorYusufoglu, Hasan
dc.contributor.authorBedir, Erdal
dc.date.accessioned2019-10-27T11:05:13Z
dc.date.available2019-10-27T11:05:13Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractA new cycloartane sapogenol and a new cycloartane xyloside were isolated from Astragalus karjaginii BORISS along with thirteen known compounds. The structures of the new compounds were established as 3-oxo-6 alpha,16 beta,24(S),25-tetrahydroxycycloartane (1) and 6-O-beta-D-xylopyranosyl-3 beta,6 alpha,16 beta,24(S),25-pentahydroxycycloartane (2) by 1D- and 2D-NMR experiments as well as ESIMS and HRMS analyses. The presence of the keto function at position 3 was reported for the first time for cyclocanthogenol sapogenin of Astragalus genus. In vitro immtmomodulatory effects of the new compounds (1 and 2) along with the n-BuOH and MeOH extracts of A. karjaginii at two different doses (3 and 6 mu g) were tested on human whole blood for in vitro cytokine release (IL-2, IL-17A and IFN-gamma) and hemolytic activities. The results confirmed that compound 2, a monodesmosidic saponin, had the strongest effect on the induction of both IL-2 (6 mu g, 6345.41 +/- 0.12 pg/mL ( x 5), P < 0.001) and a slight effect upon IL-17A (3 mu g, 5217.85 +/- 0.72 pg/mL, P < 0.05) cytokines compared to the other test compounds and positive controls (AST VII: Astragaloside VII; and QS-21: Quillaja saponin 21). All tested extracts and molecules also induced release of IFN-gamma remarkably ranging between 5031.95 +/- 0.05 pg/mL, P < 0.001 for MeOH extract (6 pg) and 5877.08 +/- 0.06 pg/mL, P < 0.001 for compound 1 (6 pg) compared to QS-21 (6 mu g, 5924.87 +/- 0.1 pg/mL, P < 0.001). Administration of AST VII and other test compounds did not cause any hemolytic activity, whereas QS-21 resulted a noteworthy hemolysis.en_US
dc.description.sponsorshipEge University BAP Research Foundation [15-FBE-001]en_US
dc.description.sponsorshipThe authors gratefully acknowledge to the financial support of Ege University BAP Research Foundation (15-FBE-001) for the financial support and thank Prof. Dr. All Asghar Maassoumi for the identification of the plant material, and special thanks to NMR operator of Prince Sattam bin Abdulaziz University titled Anzarulhaque Anwarulhaque.en_US
dc.identifier.doi10.1016/j.fitote.2017.08.008en_US
dc.identifier.endpage33en_US
dc.identifier.issn0367-326X
dc.identifier.issn1873-6971
dc.identifier.pmid28827003en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage26en_US
dc.identifier.urihttps://doi.org/10.1016/j.fitote.2017.08.008
dc.identifier.urihttps://hdl.handle.net/11454/31631
dc.identifier.volume122en_US
dc.identifier.wosWOS:000413383500006en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofFitoterapiaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAstragalus karjaginiien_US
dc.subjectSaponinsen_US
dc.subjectCycloartaneen_US
dc.subjectImmune modulationen_US
dc.subjectCytokine stimulationen_US
dc.subjectHemolytic activityen_US
dc.titleSecondary metabolites from Astragalus karjaginii BORISS and the evaluation of their effects on cytokine release and hemolysisen_US
dc.typeArticleen_US

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