Regulation of URG4/URGCP and PPAR alpha gene expressions after retinoic acid treatment in neuroblastoma cells

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dc.contributor.authorAvci, Cigir Biray
dc.contributor.authorDodurga, Yavuz
dc.contributor.authorGundogdu, Gulsah
dc.contributor.authorCaglar, Hasan Onur
dc.contributor.authorKucukatay, Vural
dc.contributor.authorGunduz, Cumhur
dc.contributor.authorSatiroglu-Tufan, N. Lale
dc.date.accessioned2019-10-27T22:06:48Z
dc.date.available2019-10-27T22:06:48Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractNeuroblastoma (NB), originating from neural crest cells, is the most common extracranial tumor of childhood. Retinoic acid (RA) which is the biological active form of vitamin A regulates differentiation of NB cells, and RA derivatives have been used for NB treatment. PPAR alpha (peroxisome proliferator-activated receptor) plays an important role in the oxidation of fatty acids, carcinogenesis, and differentiation. URG4/URGCP gene is a proto-oncogene and that overexpression of URG4/URGCP is associated with metastasis and tumor recurrence in osteosarcoma. It has been known that URG4/URGCP gene is an overexpressed gene in hepatocellular carcinoma and gastric cancers. This study aims to detect gene expression patterns of PPAR alpha and URG4/URGCP genes in SH-SY5Y NB cell line after RA treatment. Expressions levels of PPAR alpha and URG4/URGCP genes were analyzed after RA treatment for reducing differentiation in SH-SY5Y NB cell line. To induce differentiation, the cells were treated with 10 mu M RA in the dark for 3-10 days. Gene expression of URG4/URGCP and PPAR alpha genes were presented as the yield of polymerase chain reaction (PCR) products from target genes compared with the yield of PCR products from the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene. SH-SY5Y cells possess small processes in an undifferentiated state, and after treatment with RA, the cells developed long neurites, resembling a neuronal phenotype. PPAR alpha gene expression increased in RA-treated groups; URG4/URGCP gene expression decreased in SH-SY5Y cells after RA treatment compared with that in the control cells. NB cell differentiation might associate with PPAR alpha and URG4/URGCP gene expression profile after RA treatment.en_US
dc.identifier.doi10.1007/s13277-013-0970-1en_US
dc.identifier.endpage3857en_US
dc.identifier.issn1010-4283
dc.identifier.issn1423-0380
dc.identifier.issue6en_US
dc.identifier.pmid23821302en_US
dc.identifier.startpage3853en_US
dc.identifier.urihttps://doi.org/10.1007/s13277-013-0970-1
dc.identifier.urihttps://hdl.handle.net/11454/48887
dc.identifier.volume34en_US
dc.identifier.wosWOS:000328292100072en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSage Publications Ltden_US
dc.relation.ispartofTumor Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectURG4/URGCPen_US
dc.subjectPPAR alphaen_US
dc.subjectNeuroblastoma cellsen_US
dc.subjectSH-SY5Yen_US
dc.subjectDifferentiationen_US
dc.titleRegulation of URG4/URGCP and PPAR alpha gene expressions after retinoic acid treatment in neuroblastoma cellsen_US
dc.typeArticleen_US

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