Capsaicin induced apoptosis and gene expression dysregulation of human acute lymphoblastic leukemia CCRF-CEM cells
Küçük Resim Yok
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Imprimatur Publications
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Purpose: Capsaicin, an ingredient of red chili pepper, has possible tumorigenicity/genotoxicity properties. We aimed to determine the effects of capsaicin on the proliferation and gene expression profiles of acute lymphoblastic leukemia (ALL) CCRF-CEM cell line. Methods: Cell viability and IC50 dose was determined by WST cytotoxicity assay. qRT-PCR, immunohistochemical staining and western blot methods were used to determine target genes' expression levels, Apoptosis was evaluated by measuring the caspase-3 activity. Results: Capsaicin inhibited the proliferation of CCRF-CEM cells in a dose-dependent manner. Increased mRNA expressions of caspase gene family members, activated caspase-3 and decreased mRNA and protein expression of BCL-2 gene indicated apoptotic response to capsaicin. Moreover capsaicin treatment suppressed significantly the expression of the key cell signaling pathways of KRAS, AKT, GAB2, PTPN11, BRAF, INPP5D, MAPK7. Conclusion: Capsaicin induces apoptosis in CCRF-CE.M cells and this response is associated with downregulation of cell signaling pathways.
Açıklama
Anahtar Kelimeler
acute lymphoblastic leukemia, apoptosis, capsaicin, cell signaling
Kaynak
Journal of Buon
WoS Q Değeri
N/A
Scopus Q Değeri
Q3
Cilt
19
Sayı
1
