Role of intercellular communications in breast cancer multicellular tumor spheroids after chemotherapy

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dc.contributor.authorOktem, G.
dc.contributor.authorBilir, A.
dc.contributor.authorAyla, S.
dc.contributor.authorYavasoglu, A.
dc.contributor.authorGoksel, G.
dc.contributor.authorSaydam, G.
dc.contributor.authorUysal, A.
dc.date.accessioned2019-10-27T19:21:02Z
dc.date.available2019-10-27T19:21:02Z
dc.date.issued2006
dc.departmentEge Üniversitesien_US
dc.description.abstractTumor heterogeneity is an important feature that is especially involved in tumor aggressiveness. Multicellular tumor spheroids (MTS) may provide some benefits in different steps for investigation of the aggregation, organization, differentiation, and network formation of tumor cells in 3D space. This model offers a unique opportunity for improvements in the capability of a current strategy to detect the effect of an appropriate anticancer agent. The aim of this study was to investigate the cellular interactions and morphological changes following chemotherapy in a 3D breast cancer spheroid model. Distribution of the gap junction protein "connexin-43" and the tight junction protein "occludin" was investigated by immunohistochemistry. Cellular interactions were examined by using transmission and scanning electron microscopies as well as light microscopy with Giemsa staining after treating cells with doxorubicin, docetaxel, and doxorubicin/docetaxel combination. Statistical analyses showed significant changes and various alterations that were observed in all groups; however, the most prominent effect was detected in the doxorubicin/docetaxel combination group. Distinct composition as a vessel-like structure and a pseudoglandular pattern of control spheroids were detected in drug-administered groups. Immunohistochemical results were consistent with the ultrastructural changes. In conclusion, doxorubicin/docetaxel combination may be more effective than the single drug usage as shown in a 3D model. The MTS model has been found to be an appropriate and reliable method for the detection of the changes in the expression of cellular junction proteins as well as other cellular proteins occurring after chemotherapy. The MTS model can be used to validate the effects of various combinations or new chemotherapeutic agents as well as documentation of possible mechanisms of new drugs.en_US
dc.identifier.endpage233en_US
dc.identifier.issn0965-0407
dc.identifier.issue5en_US
dc.identifier.pmid17294803en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage225en_US
dc.identifier.urihttps://hdl.handle.net/11454/38943
dc.identifier.volume16en_US
dc.identifier.wosWOS:000243540800003en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherCognizant Communication Corpen_US
dc.relation.ispartofOncology Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectoccludinen_US
dc.subjectconnexin-43en_US
dc.subjectbreast cancer spheroiden_US
dc.subjectdoxorubicinen_US
dc.subjectdocetaxelen_US
dc.titleRole of intercellular communications in breast cancer multicellular tumor spheroids after chemotherapyen_US
dc.typeArticleen_US

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