Alcohol Biosensing by Polyamidoamine (PAMAM)/Cysteamine/Alcohol Oxidase-Modified Gold Electrode
Küçük Resim Yok
Tarih
2010
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A highly stable and sensitive amperometric alcohol biosensor was developed by immobilizing alcohol oxidase (A OX) through Polyamidoamine (PAMAM) dendrimers on a cysteamine-modified gold electrode surface. Ethanol determination is based on the consumption of dissolved oxygen content due to the enzymatic reaction. The decrease in oxygen level was monitored at -0.7 V vs. Ag/AgCl and correlated with ethanol concentration. Optimization of variables affecting the system was performed. The optimized ethanol biosensor showed a wide linearity from 0.025 to 1.0 mM with 100 s response time and detection limit of (LOD) 0.016 mM. In the characterization studies, besides linearity some parameters such as operational and storage stability, reproducibility, repeatability, and substrate specificity were studied in detail. Stability studies showed a good preservation of the bioanalytical properties of the sensor, 67% of its initial sensitivity was kept after 1 month storage at 4 degrees C. The analytical characteristics of the system were also evaluated for alcohol determination in flow injection analysis (FIA) mode. Finally, proposed biosensor was applied for ethanol analysis in various alcoholic beverage as well as offline monitoring of alcohol production through the yeast cultivation. (C) 2010 American Institute of Chemical Engineers Biotechnol. Prog., 26: 896-906, 2010
Açıklama
Anahtar Kelimeler
alcohol oxidase, electrochemical enzyme biosensor, dendrimer, self-assembled monolayers
Kaynak
Biotechnology Progress
WoS Q Değeri
Q1
Scopus Q Değeri
Q2
Cilt
26
Sayı
3
