An aptamer folding-based sensory platform decorated with nanoparticles for simple cocaine testing

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dc.contributor.authorGuler, Emine
dc.contributor.authorBozokalfa, Guliz
dc.contributor.authorDemir, Bilal
dc.contributor.authorGumus, Zinar Pinar
dc.contributor.authorGuler, Bahar
dc.contributor.authorAldemir, Ebru
dc.contributor.authorTimur, Suna
dc.contributor.authorCoskunol, Hakan
dc.date.accessioned2019-10-27T11:09:04Z
dc.date.available2019-10-27T11:09:04Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractThe consumption of illicit drugs such as cannabis, cocaine, and amphetamines is still a major health and social problem, creating an abuse in adults especially. Novel techniques which estimate the drug of abuse are needed for the detection of newly revealed psychoactive drugs. Herein, we have constructed a combinatorial platform by using quantum dots (QDs) and gold nanoparticles (AuNPs) as well as a functional aptamer which selectively recognizes cocaine and its metabolite benzoylecgonine (BE). We have called it an aptamer folding-based sensory device (AFSD). For the fabrication of AFSD, QDs were initially immobilized onto the poly-L-lysine coated -well surfaces. Then, the AuNP-aptamer conjugates were bound to the QDs. The addition of cocaine or BE caused a change in the aptamer structure which induced the close interaction of AuNPs with the QDs. Hence, quenching of the fluorescence of QDs was observed depending on the analyte amount. The linearity of cocaine and BE was 1.0-10 nM and 1.0-25M, respectively. Moreover, the limits of detection for cocaine and BE were calculated as 0.138 nM and 1.66M. The selectivity was tested by using different interfering substances (methamphetamine, bovine serum albumin, codeine, and 3-acetamidophenol). To investigate the use of AFSD in artificial urine matrix, cocaine/BE spiked samples were applied. Also, confirmatory analyses by using high performance liquid chromatography were performed. It is shown that AFSD has a good potential for testing the cocaine abuse and can be easily adapted for detection of various addictive drugs by changing the aptamer according to desired analytes. Copyright (c) 2016 John Wiley & Sons, Ltd.en_US
dc.description.sponsorshipRepublic of Turkey Small and Medium Enterprises Development Organization (KOSGEB)Turkiye Cumhuriyeti Kalkinma BakanligiMinistry of Science, Industry & Technology - Turkey; Industrial Thesis Support Program (SAN-TEZ) of Republic of Turkey, Ministry of Science, Industry and Technology [0620.STZ.2014]; Republic of Turkey, Ministry of DevelopmentTurkiye Cumhuriyeti Kalkinma Bakanligi [2016K121190]; Ege University Scientific Research ProjectsEge University [15-FEN-020]en_US
dc.description.sponsorshipThis work was funded by Republic of Turkey Small and Medium Enterprises Development Organization (KOSGEB) and Industrial Thesis Support Program (SAN-TEZ) of Republic of Turkey, Ministry of Science, Industry and Technology (Project Grant No: 0620.STZ.2014). Republic of Turkey, Ministry of Development was also acknowledged for their financial support (Project Grant No: 2016K121190). This study was also partially supported by Ege University Scientific Research Projects (15-FEN-020).en_US
dc.identifier.doi10.1002/dta.1992en_US
dc.identifier.endpage587en_US
dc.identifier.issn1942-7603
dc.identifier.issn1942-7611
dc.identifier.issue4en_US
dc.identifier.pmid27336666en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage578en_US
dc.identifier.urihttps://doi.org/10.1002/dta.1992
dc.identifier.urihttps://hdl.handle.net/11454/32247
dc.identifier.volume9en_US
dc.identifier.wosWOS:000398850700006en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofDrug Testing and Analysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcocaine assayen_US
dc.subjectaptasensoren_US
dc.subjectsurface modificationen_US
dc.subjectabused drugsen_US
dc.subjectbioconjugatesen_US
dc.subjectfluorescence detectionen_US
dc.titleAn aptamer folding-based sensory platform decorated with nanoparticles for simple cocaine testingen_US
dc.typeArticleen_US

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