Chitosan-ionic liquid modified single-use sensor for electrochemical monitoring of sequence-selective DNA hybridization

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dc.contributor.authorErdem, Arzum
dc.contributor.authorMuti, Mihrican
dc.contributor.authorMese, Fehmi
dc.contributor.authorEksin, Ece
dc.date.accessioned2019-10-27T22:05:23Z
dc.date.available2019-10-27T22:05:23Z
dc.date.issued2014
dc.departmentEge Üniversitesien_US
dc.description.abstractChitosan-(CHIT) and ionic liquid- (1-butyl-3-methylimidazolium hexafluorophosphate (IL)) modified single-use graphite electrodes (PGEs) were developed for the first time in the present study for the enhanced monitoring of DNA, and also for sequence-selective DNA hybridization by measuring the guanine oxidation signal. The electrochemical behaviour of the CHIT-IL modified electrodes was first investigated (with unmodified electrodes as controls) using electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). Sequence-selective DNA hybridization related to Hepatitis B virus (HBV) was also evaluated in the case of hybridization between amino-linked HBV probe and its complementary (target), a noncomplementary (NC) sequence, single base mismatch (MM), and also in the medium of target/mismatch (MM) mixtures (1:1). CHIT-IL modified PGEs presented a very effective discrimination of DNA hybridization owing to their superior selectivity and sensitivity. (C) 2013 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipTurkish Academy of Sciences (TUBA)Turkish Academy of Sciencesen_US
dc.description.sponsorshipAE acknowledges to the Turkish Academy of Sciences (TUBA) as an Associate member for their support.en_US
dc.identifier.doi10.1016/j.colsurfb.2013.10.019en_US
dc.identifier.endpage268en_US
dc.identifier.issn0927-7765
dc.identifier.issn1873-4367
dc.identifier.pmid24211827en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage261en_US
dc.identifier.urihttps://doi.org/10.1016/j.colsurfb.2013.10.019
dc.identifier.urihttps://hdl.handle.net/11454/48402
dc.identifier.volume114en_US
dc.identifier.wosWOS:000331596100035en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofColloids and Surfaces B-Biointerfacesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectChitosanen_US
dc.subjectIonic liquiden_US
dc.subject1-Butyl-3-methylimidazoliumen_US
dc.subjecthexafluorophosphateen_US
dc.subjectDNA hybridizationen_US
dc.subjectElectrochemistryen_US
dc.subjectSingle-use graphite electrodeen_US
dc.titleChitosan-ionic liquid modified single-use sensor for electrochemical monitoring of sequence-selective DNA hybridizationen_US
dc.typeArticleen_US

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