Diagnosis of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd) by RT-PCR using different extraction protocols; [Peach latent mosaic viroid (PLMVd) ve Hop stunt viroid (HSVd)’in farklı ekstraksiyon metotları kullanılarak RT-PCR ile teşhisi]

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dc.contributor.authorDuman K.
dc.contributor.authorGümüş M.
dc.date.accessioned2024-08-31T07:42:09Z
dc.date.available2024-08-31T07:42:09Z
dc.date.issued2023
dc.departmentEge Üniversitesien_US
dc.description.abstractRT-PCR method was performed using four different nucleic acid extraction methods to identify viroids; peach latent mosaic viroid (PLMVd) and hop stunt viroid (HSVd) which causes serious damage to stone fruits. Leaf samples were collected from fruit orchards showing viroid-like symptoms in İzmir province. Silica capture, citric buffer, lithium chloride, and ames buffer methods were used to extract total nucleic acids. The four extraction methods were compared using samples collected during the vegetation period from naturally infected trees (plum, apricot, and peach). They were evaluated with RT-PCR tests. In 64 stone fruit tree samples, only the silica capture method gave reliable and accurate results in RTPCR molecular tests for the detection of PLMVd and HSVd. The other wielded nucleic acid extraction methods were found to be ineffective for the isolation of the viroid RNAs. © 2023, Plant Protection Central Research Institute. All rights reserved.en_US
dc.identifier.doi10.16955/bitkorb.1239183
dc.identifier.endpage44en_US
dc.identifier.issn0406-3597
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85191354294en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage40en_US
dc.identifier.trdizinid1197319en_US
dc.identifier.urihttps://doi.org/10.16955/bitkorb.1239183
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1197319
dc.identifier.urihttps://hdl.handle.net/11454/103754
dc.identifier.volume63en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherPlant Protection Central Research Instituteen_US
dc.relation.ispartofPlant Protection Bulletin (Turkey)en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240831_Uen_US
dc.subjectnucleic aciden_US
dc.subjectRT-PCRen_US
dc.subjectstone fruiten_US
dc.subjectTNA extractionen_US
dc.subjectviroiden_US
dc.titleDiagnosis of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd) by RT-PCR using different extraction protocols; [Peach latent mosaic viroid (PLMVd) ve Hop stunt viroid (HSVd)’in farklı ekstraksiyon metotları kullanılarak RT-PCR ile teşhisi]en_US
dc.typeArticleen_US

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