Comparison of ELISA with shell vial cell culture method for the detection of human rotavirus in fecal specimens

Basit Öğe Kaydı
dc.contributor.authorCicek, Candan
dc.contributor.authorKaratas, Tuba
dc.contributor.authorAltuglu, Imre
dc.contributor.authorKoturoglu, Guldane
dc.contributor.authorKurugol, Zafer
dc.contributor.authorBilgic, Altinay
dc.date.accessioned2019-10-27T19:35:23Z
dc.date.available2019-10-27T19:35:23Z
dc.date.issued2007
dc.departmentEge Üniversitesien_US
dc.description.abstractThe aim of the study was to compare an enzyme immunoassay method with shell vial cell culture method for detection of rotavirus in fecal specimens. In addition, the correlation between laboratory results and clinical scores of patients with gastroenteritis was evaluated. A total of 219 fecal specimens from children (ages 3 weeks to 5 years) with acute gastroenteritis submitted to pediatric emergency room were evaluated by both ELISA and shell vial cell culture. A Vesikari score was used for assessing the severity of the illness. Among 219 stool samples tested, 107 (48.9%) were determined to be positive. Two specimens were positive by shell vial cell culture method while they were ELISA negative. According to these results the calculated sensitivity, specificity, PPV, and NPV of ELISA were 98.1%, 100%, 100%, and 98.2%, respectively The mean severity score for the 107 episodes of rotavirus diarrhoea was 11.0 +/- 3.6 compared to 4.5 +/- 1.9 for the 112 episodes of non-rotavirus diarrhea in the same population. Our study indicates that ELISA, which is easier to perform, faster and cheaper than cell culture methods may be suitable for routine diagnosis of rotavirus infections. The severity of rotavirus positive gastroenteritis was significantly higher than that of rotavirus negative patients.en_US
dc.identifier.endpage118en_US
dc.identifier.issn1121-7138
dc.identifier.issue2en_US
dc.identifier.pmid17619254en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage113en_US
dc.identifier.urihttps://hdl.handle.net/11454/39732
dc.identifier.volume30en_US
dc.identifier.wosWOS:000247405600006en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherEdizioni Internazionali Srlen_US
dc.relation.ispartofNew Microbiologicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectrotavirusen_US
dc.subjectshell vial cell cultureen_US
dc.subjectelisaen_US
dc.subjectclinical scoresen_US
dc.titleComparison of ELISA with shell vial cell culture method for the detection of human rotavirus in fecal specimensen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu