Aligned with sustainable development goals: microwave extraction of astaxanthin from wet algae and selective cytotoxic effect of the extract on lung cancer cells

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dc.authoridDemirel, Zeliha/0000-0003-3675-7315
dc.authoridYesil-Celiktas, Ozlem/0000-0003-4509-2212
dc.authoriddeniz, irem/0000-0002-1171-8259
dc.authorscopusid57204913357
dc.authorscopusid57204924916
dc.authorscopusid56174254800
dc.authorscopusid16202502500
dc.authorscopusid22837401200
dc.authorscopusid16203196800
dc.authorwosidImamoglu, Esra/ABB-5532-2020
dc.authorwosidYesil-Celiktas, Ozlem/AAI-5713-2020
dc.authorwosidDemirel, Zeliha/AAG-4733-2019
dc.authorwosidGuler, Bahar Aslanbay/AAA-4768-2022
dc.contributor.authorGuler, Bahar Aslanbay
dc.contributor.authorSaglam-Metiner, Pelin
dc.contributor.authorDeniz, Irem
dc.contributor.authorDemirel, Zeliha
dc.contributor.authorYesil-Celiktas, Ozlem
dc.contributor.authorImamoglu, Esra
dc.date.accessioned2023-01-12T19:59:41Z
dc.date.available2023-01-12T19:59:41Z
dc.date.issued2022
dc.departmentN/A/Departmenten_US
dc.description.abstractAstaxanthin is one of the most attractive carotenoid in the cosmetic, food, pharmaceutical, and aquaculture industries due to its strong bioactive properties. Among the various sources, several algae species are considered as rich sources of astaxanthin. Downstream processing of algae involves the majority of the total processing costs. Thus, elimination of high energy involved steps is imperative to achieve cost-effective scale in industry. This study aimed to determine operation conditions for astaxanthin extraction from wet Haematococcus pluvialis using microwave-assisted extraction. The isolated astaxanthin extract was evaluated for cytotoxicity on human lung cancer cells. The microwave-assisted extraction process at 75 degrees C under the power of 700 Watt for 7 min gave the highest astaxanthin yield (12.24 +/- 0.54 mg astaxanthin/g wet cell weight). Based on MTT cell viability and Hoechst 33342 nuclear staining assays on A549 lung cancer cells, astaxanthin inhibited cell growth in dose- and time-dependent manners, where IC50 value was determined as 111.8 +/- 14.8 mu g/mL and apoptotic bodies were observed along with positive control group at 72 hr. These results showed that the treatment with astaxanthin extracted from wet H. pluvialis by microwave-assisted extraction exhibited anti-cancer activity on lung cancer cells indicating a newly potential to be utilized in industry.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) part of Cost Action [115M014, ES1408]en_US
dc.description.sponsorshipThis study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) with the project number of 115M014 as a part of Cost Action ES1408.en_US
dc.identifier.doi10.1080/10826068.2022.2116455
dc.identifier.issn1082-6068
dc.identifier.issn1532-2297
dc.identifier.pmid36047960en_US
dc.identifier.scopus2-s2.0-85137985626en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.urihttps://doi.org/10.1080/10826068.2022.2116455
dc.identifier.urihttps://hdl.handle.net/11454/77216
dc.identifier.wosWOS:000848796000001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofPreparative Biochemistry & Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAstaxanthinen_US
dc.subjectHaematococcus pluvialisen_US
dc.subjectwet biomassen_US
dc.subjectmicrowave-assisted extractionen_US
dc.subjectlung canceren_US
dc.subjectMTTen_US
dc.subjectResponse-Surface Methodologyen_US
dc.subjectHaematococcus-Pluvialisen_US
dc.subjectAssisted Extractionen_US
dc.titleAligned with sustainable development goals: microwave extraction of astaxanthin from wet algae and selective cytotoxic effect of the extract on lung cancer cellsen_US
dc.typeArticleen_US

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