Effect of complete hilar versus only renal artery clamping on renal histomorphology following ischemia/reperfusion injury in an experimental model

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dc.contributor.authorUmul, M.
dc.contributor.authorCal, A. C.
dc.contributor.authorTurna, B.
dc.contributor.authorOktem, G.
dc.contributor.authorAydin, H. H.
dc.date.accessioned2019-10-27T23:11:27Z
dc.date.available2019-10-27T23:11:27Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractOBJECTIVE: To evaluate the effect of temporary complete hilar versus only renal artery clamping with different duration of warm ischemia on renal functions, and possibly identify a "safe" clamping type and duration of renal ischemia. MATERIALS AND METHODS: Fifty male rabbits have been incorporated to study. Rabbits were subjected to ischemia/reperfusion injury by temporary vascular clamping. Reagents were randomized to 3 experimental groups (only renal artery clamping, complete hilar clamping, sham surgery) and sub-groups were determined according to different clamping times (30 and 60 minutes). Median laparotomy and left renal hilus dissection were performed to sham group. Only artery or complete hilar clamping was performed for 30 or 60 minutes by microvascular bulldog clamps to other reagents. Rabbits were sacrificed 10 days after primary surgery and left nephrectomy performed. Nephrectomy materials were evaluated for the level of nitric-oxide synthase (NOS) immunoreactivity, malondialdehyde (MDA) level and superoxide dismutase (SOD) activity and an electron microscopic examination was performed. RESULTS: NOS immunoreactivity was correlated with the temporary clamping time. We also observed that complete hilar vascular clamping entails an increase on NOS immunoreactivity. MDA levels were similar for all experimental surgery groups (p = 0.42). The SOD activity was decreased among all subgroups compared with sham surgery. But the significant decrease occurred in 30 minutes only artery and 30 minutes complete hilar clamping groups in proportion to sham surgery (p = 0.026 and p = 0.019, respectively). CONCLUSIONS: This current study suggested that only renal artery clamping under 30 minutes is more appropriate during renal surgical procedures requiring temporary vascular clamping.en_US
dc.description.sponsorshipAcademic Research Projects Unit of the University of EgeEge Universityen_US
dc.description.sponsorshipThis study was supported by the Academic Research Projects Unit of the University of Ege.en_US
dc.identifier.endpage597en_US
dc.identifier.issn1128-3602
dc.identifier.issue4en_US
dc.identifier.pmid26957258en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage589en_US
dc.identifier.urihttps://hdl.handle.net/11454/53079
dc.identifier.volume20en_US
dc.identifier.wosWOS:000373349200003en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherVerduci Publisheren_US
dc.relation.ispartofEuropean Review For Medical and Pharmacological Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectTemporary renal vascular clampingen_US
dc.subjectIschemia/reperfusion injuryen_US
dc.subjectNephron-sparing surgeryen_US
dc.subjectNitric oxide synthase immunoreactivityen_US
dc.subjectSuperoxide dismutase activityen_US
dc.subjectMalondialdehyde levelen_US
dc.titleEffect of complete hilar versus only renal artery clamping on renal histomorphology following ischemia/reperfusion injury in an experimental modelen_US
dc.typeArticleen_US

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