Doku mühendisliğinde kullanıma uygun nanoliflerin elektroeğirme ile üretimi ve karakterizasyonu
Küçük Resim Yok
Dosyalar
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Ege Üniversitesi, Fen Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, elektroeğirmeyle üretilen nanoliflerden deri doku mühendisliğinde kullanılmak amacıyla doku iskelesi ve yara örtüsü geliştirildi. Deri doku mühendisliğinde yara iyileşme uygulamalarında kullanılmak amacıyla poli(ε-kaprolakton) (PCL), Κ-karragenan (Kar) ve Nigella sativa tohum yağı (NSO) kullanılarak nanolifler üretildi. PCL-KAR-ÇOY nanoliflerinin hücre canlılığı üzerine etkileri DAPI kullanılarak floresans mikroskop yardımıyla belirlenirken, yara iyileşme aktivitelerinin araştırılmasında culture insertler kullanıldı. 10 mg/mL çörek otu yağı içeren PCL-Kar-ÇOY nanolifleri kontrole kıyasla 48 ve 72 saat boyunca sırasıyla %43,16±15,56 ve %48,33±14,72 yara iyileşme aktivitesi göstererek daha etkili bulundu. Nanolif temelli biyoaktif yara örtülerinin geliştirilmesi amacıyla PCL, selüloz asetat (CA) ve mandelik asit (MA) kullanıldı. PCL-CA-MA nanoliflerinin HaCaT hücre canlılığına etkileri MTT testi ile belirlendi. 25 mM MA içeren PCL-CA nanolifleri en etkin konsantrasyon olarak belirlendi. PCL-CA-MA (25 mM) nanolifleri (sırasıyla 4, 6 ve 8 saat için %39,35±12,46; %65,31±10,74 ve %94,78±6,77) kontrol grubuna (sırasıyla 4, 6 ve 8 saat için %17,88±3,34; %19,36±6,64 ve %29,07±7,66) kıyasla çok kısa sürede oldukça yüksek bir yara iyileşme aktivitesi gösterdi.
In this study, electrospun nanofibers were developed for use in the field of skin tissue engineering as scaffold and wound dressing material. Nanofibers were prepared with poly(ε-caprolactone) (PCL), Κ-carrageenan (Car) and Nigella sativa seed oil (NSO) for the purpose of developing the skin tissue engineering matrices for wound healing. Cell viability experiments were performed on HaCaT cell line for the choosing of appropriate PCL-Car/NSO nanofibers via fluorescence microscopy with DAPI, and wound healing activity was investigated with culture inserts performed. 10 mg/mL NSO containing PCL-Car/NSO nanofibers were found more effective at wound healing compared with the control 43.16 ± 15.56% and 48.33 ± 14.72% for 48 and 72 hours, respectively. PCL, cellulose acetate (CA) and mandelic acid (MA) were used for the development of nanofibrous bioactive wound dressing material. PCL-CA-MA nanofibers’ effects on viability of HaCaT cell lines were determined by MTT. PCL-CA nanofibers, which contained 25 mM MA, were found the most effective nanofiber formulation. PCL-CA-MA (25 mM) nanofibers showed an excellent wound healing activity (39.35±12.46%; 65.31±10.74% and 94.78±6.77% for 4, 6 and 8 hours, respectively) compared with control group, (17.88±3.34%; 19.36±6.64% and 29.07±7.66% for 4, 6 and 8 hours, respectively) in a very short time.
In this study, electrospun nanofibers were developed for use in the field of skin tissue engineering as scaffold and wound dressing material. Nanofibers were prepared with poly(ε-caprolactone) (PCL), Κ-carrageenan (Car) and Nigella sativa seed oil (NSO) for the purpose of developing the skin tissue engineering matrices for wound healing. Cell viability experiments were performed on HaCaT cell line for the choosing of appropriate PCL-Car/NSO nanofibers via fluorescence microscopy with DAPI, and wound healing activity was investigated with culture inserts performed. 10 mg/mL NSO containing PCL-Car/NSO nanofibers were found more effective at wound healing compared with the control 43.16 ± 15.56% and 48.33 ± 14.72% for 48 and 72 hours, respectively. PCL, cellulose acetate (CA) and mandelic acid (MA) were used for the development of nanofibrous bioactive wound dressing material. PCL-CA-MA nanofibers’ effects on viability of HaCaT cell lines were determined by MTT. PCL-CA nanofibers, which contained 25 mM MA, were found the most effective nanofiber formulation. PCL-CA-MA (25 mM) nanofibers showed an excellent wound healing activity (39.35±12.46%; 65.31±10.74% and 94.78±6.77% for 4, 6 and 8 hours, respectively) compared with control group, (17.88±3.34%; 19.36±6.64% and 29.07±7.66% for 4, 6 and 8 hours, respectively) in a very short time.
Açıklama
Anahtar Kelimeler
Biyomalzeme, Doku Mühendisliği, Nanobiyoteknoloji, Nanolif, Poli(ε-Kaprolakton), K-Karragenan, Nigella Sativa, Selüloz Asetat, Mandelik Asit, Biomaterial, Tissue Engineering, Nanobiotechnology, Nanofiber, Poly(ε-Caprolactone), K-Carrageenan, Nigella Sativa, Cellulose Acetate, Mandelic Acid
