A new insight into electrochemical microRNA detection: A molecular caliper, p19 protein

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dc.contributor.authorKilic, Tugba
dc.contributor.authorTopkaya, Seda Nur
dc.contributor.authorOzsoz, Mehrnet
dc.date.accessioned2019-10-27T22:07:36Z
dc.date.available2019-10-27T22:07:36Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractmicroRNA (miRNA) has drawn a great attention in biomedical research due to its functions on biological processes. Detection of miRNAs is a big challenge since the amount present in real samples is very low and the length of them is short. In this study, for the first time an electrochemical biosensor for detection of mir21 using the oxidation signal of protein 19 (p19) as a molecular caliper was designed. The proposed method enables detection of mir21 in direct, rapid, sensitive, inexpensive and label-free way. Binding specificity of the p19 to 20-23 base pair length double stranded RNA (dsRNA) and direct/water-mediated intermolecular contacts between the fusion protein and miRNA allows detection of miRNA-antimiRNA hybrid structure. The detection of mir21 was achieved in picomole sensitivity through the changes of intrinsic p19 oxidation signals observed at +0.80 V with Differential Pulse Voltammetry (DPV) and the specifity of the designed sensor was proved by control studies. (C) 2013 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.bios.2013.04.011en_US
dc.identifier.endpage171en_US
dc.identifier.issn0956-5663
dc.identifier.issn1873-4235
dc.identifier.pmid23680935en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage165en_US
dc.identifier.urihttps://doi.org/10.1016/j.bios.2013.04.011
dc.identifier.urihttps://hdl.handle.net/11454/49042
dc.identifier.volume48en_US
dc.identifier.wosWOS:000321085600027en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Advanced Technologyen_US
dc.relation.ispartofBiosensors & Bioelectronicsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectp19 proteinen_US
dc.subjectmir21en_US
dc.subjectElectrochemical DNA biosensoren_US
dc.subjectDifferential pulse voltammetry (DPV)en_US
dc.subjectBreast canceren_US
dc.titleA new insight into electrochemical microRNA detection: A molecular caliper, p19 proteinen_US
dc.typeArticleen_US

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