Comparative Cytochrome P450-1A1, -2A6, -2B6, -2C, -2D6, -2E1, -3A5 and -4B1 expressions in human larynx tissue analysed at mRNA level

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dc.contributor.authorSarikaya, Devrim
dc.contributor.authorBilgen, Cem
dc.contributor.authorKamataki, Tetsuya
dc.contributor.authorTopcu, Zeki
dc.date.accessioned2019-10-27T19:40:23Z
dc.date.available2019-10-27T19:40:23Z
dc.date.issued2006
dc.departmentEge Üniversitesien_US
dc.description.abstractThe metabolic activation of numerous exogenous and endogenous chemicals is catalysed by cytochrome P450 enzymes (CYPs). The aim of this study was to analyse the expression of the individual forms of CYP at the mRNA level in human larynx and quantitatively to compare their expressions in human liver, the main in organ of CYP expression. Individual forms of CYP mRNAs were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) using specific primers for the CYPs -1A1, -1A2, -2A6,-2B6,-2C, -2D6, -2E1, -3A3/4 ,-3A5, -3A7 and 4B1. An RNA competitor of known copy number, covering the primer sequences necessary to amplify the entire object CYPs within a single molecule, was used as reference. This study reports a consistent detection of mRNAs for the CYPs -1A1, -2A6, -2B6, -2C, -2D6, -2E1, -3A5 and -4B1 in the human larynx tissue. The data indicate that the human larynx highly resembles the lung tissue in CYP content, as a comparable subset of CYP mRNAs was detected in the larynx previously reported for human lung with the exception of CYP1A2. The results are discussed in quantitative ratios of the detected CYP mRNAs in relation to the hepatic CYP expression. Copyright (c) 2006 John Wiley & Sons, Ltd.en_US
dc.identifier.doi10.1002/bdd.518en_US
dc.identifier.endpage359en_US
dc.identifier.issn0142-2782
dc.identifier.issue8en_US
dc.identifier.pmid16894644en_US
dc.identifier.startpage353en_US
dc.identifier.urihttps://doi.org/10.1002/bdd.518
dc.identifier.urihttps://hdl.handle.net/11454/40259
dc.identifier.volume27en_US
dc.identifier.wosWOS:000242307900001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherJohn Wiley & Sons Ltden_US
dc.relation.ispartofBiopharmaceutics & Drug Dispositionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcytochrome P450en_US
dc.subjectlaryngeal tissueen_US
dc.subjectdrug metabolizing enzymesen_US
dc.titleComparative Cytochrome P450-1A1, -2A6, -2B6, -2C, -2D6, -2E1, -3A5 and -4B1 expressions in human larynx tissue analysed at mRNA levelen_US
dc.typeArticleen_US

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