Endometriozis ve immünite: Kompleman sistem proteinlerinin rolü ve kompleman sistem aktivasyonunun incelenmesi
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Dosyalar
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Ege Üniversitesi, Sağlık Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Endometriozis, üreme çağındaki kadınları etkileyen jinekolojik bir hastalıktır. Hastalığın etiyolojisi ve patolojisi tam olarak aydınlatılmamıştır. Hastalığın temellerini anlamaya yönelik güncel çalışmalar, bağışıklık sistemi proteinleri ile ilişkili teorileri desteklemektedir. Hastalık süresince kompleman sistem proteinlerinin gen ekspresyon profilinde anlamlı ölçüde bir değişim olduğu daha önceki çalışmalarda gösterilmiştir. Bu nedenle kompleman sistem proteinleri tanısal ve/veya prognostik amaçlar için iyi birer biyobelirteç olabilme özelliği taşımaktadır. Bu çalışmada, aday kompleman sistem proteinlerini, serum düzeylerindeki değişimlerini baz alarak, endometriozis için potansiyel birer klinik biyobelirteç olarak değerlendirdik. Bu tezde, tıbbi sebeplerle opere edilmesine karar verilen 71 endometriozis vakasından ve aynı yaş aralığındaki endometriozis tanısı almamış 79 kadından kan örneği alınmıştır. Hasta grubundan operasyon öncesi ve sonrasında alınan örneklerde, kompleman sistem proteinleri C3, C5 ve C6 serum düzeyleri ölçülmüştür. Endometriozis tanısı olmayan kontrol grubunda serum C3, C5 ve C6 düzeyleri hasta grubu ile karşılaştırılmıştır. Sonuçlar, daha önceki çalışmamızda dokudaki mRNA düzeyinde C5 kompleman proteini için gözlenen değişikliğin, serum protein düzeylerine yansımadığını göstermektedir. Kompleman yolağında C5 proteininin akışyukarısında yer alan C3 proteini için de benzer bulgulara ulaşılmıştır. Buna karşın, operasyon sonrası C6 düzeylerinin, endometriozis tanısı almamış kadınlara göre anlamlı artış gösterdiği, bu tez ile ortaya konan yeni bir bulgudur.
Endometriosis is a gynecological disease, affecting women in the reproductive ages. The etiology and pathology of the disease have not been completely elucidated. Current studies to understand the basis of the disease support the theories associated with the involvement of immune response proteins. In previous studies, it was shown that complement system proteins have a dramatic change in their gene expression profiles during the course of the disease. Therefore, complement system proteins can be good biomarkers for diagnostic and/or prognostic purposes. In this study, we evaluated candidate complement system proteins as potential clinical biomarkers of endometriosis based on their serum level changes. In this thesis, blood samples from 71 endometriosis cases with the decision of operation due to medical reasons and from 79 women without endometriosis within the matching age range were taken. Serum levels of complement system proteins, C3, C5 and C6 were measured in the samples taken from the patient group before and after the operations. Serum levels of C3, C5 and C6 in the control group who were not diagnosed with endometriosis, were compared with the patient group. Results showed that the change observed for C5 complement protein at the tissue mRNA level in the previous study of our working group was not reflected in serum protein levels. Similar results were obtained for C3 protein, which functions as an upstream factor of C5 in the complement pathway. In spite of that, discovery of a significant difference between C6 levels measured in postoperative samples of endometriosis patients and women without endometriosis is a new finding revealed with the current thesis.
Endometriosis is a gynecological disease, affecting women in the reproductive ages. The etiology and pathology of the disease have not been completely elucidated. Current studies to understand the basis of the disease support the theories associated with the involvement of immune response proteins. In previous studies, it was shown that complement system proteins have a dramatic change in their gene expression profiles during the course of the disease. Therefore, complement system proteins can be good biomarkers for diagnostic and/or prognostic purposes. In this study, we evaluated candidate complement system proteins as potential clinical biomarkers of endometriosis based on their serum level changes. In this thesis, blood samples from 71 endometriosis cases with the decision of operation due to medical reasons and from 79 women without endometriosis within the matching age range were taken. Serum levels of complement system proteins, C3, C5 and C6 were measured in the samples taken from the patient group before and after the operations. Serum levels of C3, C5 and C6 in the control group who were not diagnosed with endometriosis, were compared with the patient group. Results showed that the change observed for C5 complement protein at the tissue mRNA level in the previous study of our working group was not reflected in serum protein levels. Similar results were obtained for C3 protein, which functions as an upstream factor of C5 in the complement pathway. In spite of that, discovery of a significant difference between C6 levels measured in postoperative samples of endometriosis patients and women without endometriosis is a new finding revealed with the current thesis.
Açıklama
Anahtar Kelimeler
Endometriozis, Bağışıklık Sistemi, Kompleman Sistem, Biyobelirteç, Endometriosis, Immune System, Complement System, Biomarker
