Differences and similarities in biophysical and biological characteristics between U87 MG glioblastoma and astrocyte cells

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dc.authoridGÖRGÜLÜ, Volkan/0000-0003-4026-2972
dc.authoridUnsalan, Ozan/0000-0001-5736-7530
dc.authoridAcikgoz, Eda/0000-0002-6772-3081
dc.authoridAltunayar-Unsalan, Cisem/0000-0001-6479-4223
dc.authorscopusid56301503200
dc.authorscopusid57226366079
dc.authorscopusid57201338243
dc.authorscopusid56364984200
dc.authorscopusid55318033800
dc.authorscopusid57226354334
dc.authorscopusid7005410144
dc.authorwosidGÖRGÜLÜ, Volkan/JJF-9615-2023
dc.authorwosidUnsalan, Ozan/AAG-4343-2019
dc.contributor.authorÖzdil, Berrin
dc.contributor.authorÇalık-Kocatürk, Duygu
dc.contributor.authorAltunayar-Unsalan, Cisem
dc.contributor.authorAcikgoz, Eda
dc.contributor.authorOltulu, Fatih
dc.contributor.authorGörgülü, Volkan
dc.contributor.authorUysal, Ayşegül
dc.date.accessioned2024-08-25T18:36:52Z
dc.date.available2024-08-25T18:36:52Z
dc.date.issued2023
dc.departmentEge Üniversitesien_US
dc.description.abstractCurrent cancer studies focus on molecular-targeting diagnostics and interactions with surroundings; however, there are still gaps in characterization based on topological differences and elemental composition. Glioblastoma (GBM cells; GBMCs) is an astrocytic aggressive brain tumor. At the molecular level, GBMCs and astrocytes may differ, and cell elemental/topological analysis is critical for identifying potential new cancer targets. Here, we used U87 MG cells for GBMCS. U87 MG cell lines, which are frequently used in glioblastoma research, are an important tool for studying the various features and underlying mechanisms of this aggressive brain tumor. For the first time, atomic force microscopy (AFM), scanning electron microscopy (SEM) accompanied by energy-dispersive X-ray spectroscopy (EDS), and X-ray photoelectron spectroscopy (XPS) are used to report the topology and chemistry of cancer (U87 MG) and healthy (SVG p12) cells. In addition, F-actin staining and cytoskeleton-based gene expression analyses were performed. The degree of gene expression for genes related to the cytoskeleton was similar; however, the intensity of F-actin, anisotropy values, and invasion-related genes were different. Morphologically, GBMCs were longer and narrower while astrocytes were shorter and more disseminated based on AFM. Furthermore, the roughness values of these cells differed slightly between the two call types. In contrast to the rougher astrocyte surfaces in the lamellipodial area, SEM-EDS analysis showed that elongated GBMCs displayed filopodial protrusions. Our investigation provides considerable further insight into rapid cancer cell characterization in terms of a combinatorial spectroscopic and microscopic approach.en_US
dc.description.sponsorshipEge University Scientific Research Projects Coordination Unit [18-TIP-013, 117S578]; TUBITAK [3001]en_US
dc.description.sponsorshipThis study was supported by Ege University Scientific Research Projects Coordination Unit. Project Number: 18-TIP-013 and 117S578 TUBITAK (3001) project.en_US
dc.identifier.doi10.1007/s00418-023-02234-0
dc.identifier.issn0948-6143
dc.identifier.issn1432-119X
dc.identifier.pmid37700206en_US
dc.identifier.scopus2-s2.0-85171182467en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1007/s00418-023-02234-0
dc.identifier.urihttps://hdl.handle.net/11454/100783
dc.identifier.wosWOS:001064523800001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofHistochemistry and Cell Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240825_Gen_US
dc.subjectGBMen_US
dc.subjectAstrocytesen_US
dc.subjectAFMen_US
dc.subjectSEM/EDSen_US
dc.subjectXPSen_US
dc.subjectCentral-Nervous-Systemen_US
dc.subjectCancer-Cellsen_US
dc.subjectQuantitative-Analysisen_US
dc.subjectRho-Gtpasesen_US
dc.subjectForceen_US
dc.subjectMicroscopyen_US
dc.subjectDiscriminationen_US
dc.subjectElasticityen_US
dc.subjectAdhesionen_US
dc.subjectSurfaceen_US
dc.titleDifferences and similarities in biophysical and biological characteristics between U87 MG glioblastoma and astrocyte cellsen_US
dc.typeArticleen_US

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