Diagnosis and Species Discrimination of Positive Malaria Samples by Real-Time Polymerase Chain Reaction

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dc.contributor.authorAtalay Şahar E.
dc.contributor.authorKarakavuk M.
dc.contributor.authorCan H.
dc.contributor.authorNergiz Ş.
dc.contributor.authorGül K.
dc.contributor.authorDeğirmenci Döşkaya A.
dc.contributor.authorGürüz Y.
dc.contributor.authorDöşkaya M.
dc.date.accessioned2019-10-26T21:18:13Z
dc.date.available2019-10-26T21:18:13Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractOBJECTIVE: Malaria is an important tropical disease that is detected in 198 million people and causes 367-755 thousand deaths annually. Recently, the real-time Polymerase Chain Reaction (PCR) technique has enabled quick determination of Plasmodium spp. and species identification in the same assay with a low contamination risk. In the present study, we aimed to use real-time PCR targeting the 18S rRNA gene to diagnose Plasmodium spp. and perform species identification.METHODS: DNA samples of 15 patients with malaria (14 caused by P. vivax, 1 caused by P. falciparum) confirmed by microscopy as well as positive control plasmids were used. As the negative control, DNA samples of 15 individuals without malaria were used.RESULTS: According to the results of real-time PCR, samples of 15 patients with malaria were found to be positive for Plasmodium spp. Melting curve analysis showed that 14 of them were P. vivax and the remaining was P. falciparum. In addition, mixed infection with P. falciparum and P. vivax was successfully detected by real-time PCR when DNA of P. falciparum- and P. vivax-positive samples was experimentally mixed.CONCLUSION: The present study showed that real-time PCR can be useful in the diagnosis and species identification of Plasmodium spp. as well as the detection of mixed infections in addition to microscopy in Turkey.en_US
dc.identifier.doi10.5152/tpd.2016.4711en_US
dc.identifier.endpage131en_US
dc.identifier.issn2146-3077
dc.identifier.issue3en_US
dc.identifier.pmid27905280en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage126en_US
dc.identifier.urihttps://doi.org/10.5152/tpd.2016.4711
dc.identifier.urihttps://hdl.handle.net/11454/16485
dc.identifier.volume40en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofTurkiye parazitolojii dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleDiagnosis and Species Discrimination of Positive Malaria Samples by Real-Time Polymerase Chain Reactionen_US
dc.typeArticleen_US

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