Karpuz bakteriyel meyve lekesi hastalığı etmeni Acidovorax citrulli'nin moleküler tanısı ve endofitik bakterilerle önlenme olanaklarının araştırılması
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Dosyalar
Tarih
2016
Yazarlar
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Yayıncı
Ege Üniversitesi, Fen Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu tez çalışmasının amacı, Acidovorax citrulli (Ac)'nin neden olduğu karpuz bakteriyel meyve lekesi hastalığına karşı endofitik bakterilerin (EB) etkisinin araştırılması ve Ac'nin moleküler tanısının gerçekleştirilmesidir. Sağlıklı hıyar bitkilerinden izole edilmiş 20 EB izolatı Ac'ye karşı in vivo koşullarda antagonistik aktivite açısından testlenmiştir. Vakum infiltrasyon yöntemiyle Ac ile inokule edilmiş karpuz tohumlarına EB süspansiyonu uygulanmıştır. Bakıroksiklorid ve mancozeb etkili maddeli preparatlar da referans kimyasallar olarak kullanılmıştır. Uygulama görmüş karpuz tohumları büyüme odasında tutulmuş; fide çıkışı ve kotiledon yapraklardaki hastalık belirtilerine göre değerlendirme yapılmıştır. In vivo test sonuçlarına göre; EB izolatlarının % 50'den fazlası pozitif kontrolle karşılaştırıldığında % 64-85 oranında karpuz fidelerinin kotiledonlarında hastalık gelişimini engellemiş ve referans kimyasallardan daha etkili bulunmuştur. Ac'ye karşı in vivo'da başarılı bulunan EB izolatlarının olası in vitro etki mekanizmasının Fe+3 için yarışmaya dayalı olduğu saptanmıştır. Ac'ye etkili bulunan EB izolatları, 16s rRNA primerleri ile yapılan sekans analizi sonucunda; Pseudomonas fluorescens (31 no.lu izolat), P. lutea (53 no.lu izolat), P. putida (61 no.lu izolat), Enterobacter cloaceae (108 no.lu izolat), Chryseobacterium elymi (88 no.lu izolat), Cronobacter sakazakii (15 no.lu izolat) ve Serratia ureilytica (18 no.lu izolat) olarak tanılanmıştır.
The aim of this thesis was to evaluate the effect of endophytic bacterial (EB) strains on bacterial fruit blotch of watermelon caused by Acidovorax citrulli (Ac), and to realize molecular identification of Ac. Out of 20 EB isolates, which were isolated from internal tissues of healthy cucumber plants, were screened for in vivo antagonistic activity against Ac. Watermelon seeds, which were previously inoculated with Ac suspension by vacuum infiltration technique were treated by EB suspensions. Copper oxychloride and mancozeb were used as reference chemicals. Treated watermelon seeds were sown in peat medium and kept in growth chamber room, and then evaluated for seedling emergence and disease symptoms on cotyledone leaves. According to in vivo test results, more than 50% of tested EB isolates inhibited the disease development on cotyledons of seedlings at the rate of 64 to 85% compared to pathogen alone treatment, and found more effective than tested chemicals. It was also determined that the possible mode of action of EB strains to Ac was based on competition for Fe 3+. The most effective EB strains were identified as Pseudomonas fluorescens (strain 31), P. lutea (strain 53), P. putida (strain 61), Enterobacter cloaceae (strain 108), Chryseobacterium elymi (strain 88), Cronobacter sakazakii (strain 15) and Serratia ureilytica (strain 18) by using 16s rRNA primers and sequence analysis.
The aim of this thesis was to evaluate the effect of endophytic bacterial (EB) strains on bacterial fruit blotch of watermelon caused by Acidovorax citrulli (Ac), and to realize molecular identification of Ac. Out of 20 EB isolates, which were isolated from internal tissues of healthy cucumber plants, were screened for in vivo antagonistic activity against Ac. Watermelon seeds, which were previously inoculated with Ac suspension by vacuum infiltration technique were treated by EB suspensions. Copper oxychloride and mancozeb were used as reference chemicals. Treated watermelon seeds were sown in peat medium and kept in growth chamber room, and then evaluated for seedling emergence and disease symptoms on cotyledone leaves. According to in vivo test results, more than 50% of tested EB isolates inhibited the disease development on cotyledons of seedlings at the rate of 64 to 85% compared to pathogen alone treatment, and found more effective than tested chemicals. It was also determined that the possible mode of action of EB strains to Ac was based on competition for Fe 3+. The most effective EB strains were identified as Pseudomonas fluorescens (strain 31), P. lutea (strain 53), P. putida (strain 61), Enterobacter cloaceae (strain 108), Chryseobacterium elymi (strain 88), Cronobacter sakazakii (strain 15) and Serratia ureilytica (strain 18) by using 16s rRNA primers and sequence analysis.
Açıklama
Anahtar Kelimeler
Karpuz Bakteriyel Meyve Lekesi, Acidovorax Citrulli, Biyolojik Mücadele, Endofitik Bakteriler, Moleküler Tanı, Bacterial Fruit Blotch Of Watermelon, Acidovorax Citrulli, Biological Control, Endophytic Bacteria, Molecular Diagnosis
