Alkaline protease production by an actinomycete MA1-1 isolated from marine sediments

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dc.contributor.authorHames-Kocabas, E. Esin
dc.contributor.authorUzel, Atac
dc.date.accessioned2019-10-27T19:37:02Z
dc.date.available2019-10-27T19:37:02Z
dc.date.issued2007
dc.departmentEge Üniversitesien_US
dc.description.abstractAlkaliphilic actinomycetes isolated from sediment samples of the Izmir Gulf, Turkey were studied for the production of protease activity. Strain MA1-1 was selected as a good alkaline protease producer as measured by the clear zone diameter by the hydrolysis of skim-milk and casein. The alkaline protease production from the marine alkaliphilic actinomycete MA1-1 was studied by using different carbon and nitrogen sources in medium containing glycerol, peptone, KCl, MgSO4, K2HPO4, and trace elements at 30 degrees C for 72 h. Among the different carbon and nitrogen sources, fructose, starch, maltose, D(+) glucose, yeast extract, malt extract, beef extract and peptone provided higher production of protease. Starch was also found to be effective for growth and enzyme production with highest specific activity at 699 U mg(-1). Purification was achieved by adsorption on Diaion HP 20 which resulted in a recovery rate of 68% with a specific activity of 7618 U mg(-1) protein and 40-fold purification. The optimum pH and temperature of the partially purified protease were determined as pH 9.0 and 50 degrees C, but high activity was also observed at pH 8.0-13.0 and 35-50 degrees C. The inhibition profile exhibited by phenylmethylsulphonyl fluoride (PMSF) showed that this enzyme belongs to the serine-protease group.en_US
dc.identifier.doi10.1007/BF03175053
dc.identifier.endpage75en_US
dc.identifier.issn1590-4261
dc.identifier.issn1869-2044
dc.identifier.issn1590-4261en_US
dc.identifier.issn1869-2044en_US
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage71en_US
dc.identifier.urihttps://doi.org/10.1007/BF03175053
dc.identifier.urihttps://hdl.handle.net/11454/39925
dc.identifier.volume57en_US
dc.identifier.wosWOS:000245462200012en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofAnnals of Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectalkaline proteaseen_US
dc.subjectalkaliphilicen_US
dc.subjecthalophilicen_US
dc.subjectactinomyceteen_US
dc.subjectmarine sedimentsen_US
dc.titleAlkaline protease production by an actinomycete MA1-1 isolated from marine sedimentsen_US
dc.typeArticleen_US

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