Alkaline protease production by an actinomycete MA1-1 isolated from marine sediments
Küçük Resim Yok
Tarih
2007
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Alkaliphilic actinomycetes isolated from sediment samples of the Izmir Gulf, Turkey were studied for the production of protease activity. Strain MA1-1 was selected as a good alkaline protease producer as measured by the clear zone diameter by the hydrolysis of skim-milk and casein. The alkaline protease production from the marine alkaliphilic actinomycete MA1-1 was studied by using different carbon and nitrogen sources in medium containing glycerol, peptone, KCl, MgSO4, K2HPO4, and trace elements at 30 degrees C for 72 h. Among the different carbon and nitrogen sources, fructose, starch, maltose, D(+) glucose, yeast extract, malt extract, beef extract and peptone provided higher production of protease. Starch was also found to be effective for growth and enzyme production with highest specific activity at 699 U mg(-1). Purification was achieved by adsorption on Diaion HP 20 which resulted in a recovery rate of 68% with a specific activity of 7618 U mg(-1) protein and 40-fold purification. The optimum pH and temperature of the partially purified protease were determined as pH 9.0 and 50 degrees C, but high activity was also observed at pH 8.0-13.0 and 35-50 degrees C. The inhibition profile exhibited by phenylmethylsulphonyl fluoride (PMSF) showed that this enzyme belongs to the serine-protease group.
Açıklama
Anahtar Kelimeler
alkaline protease, alkaliphilic, halophilic, actinomycete, marine sediments
Kaynak
Annals of Microbiology
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
57
Sayı
1
