A new method for studying the adhesion of Candida albicans to dentin in the presence or absence of smear layer

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dc.contributor.authorHakan, B
dc.contributor.authorChugal, NM
dc.contributor.authorLiu, H
dc.contributor.authorFleischmann, J
dc.date.accessioned2019-10-27T18:59:45Z
dc.date.available2019-10-27T18:59:45Z
dc.date.issued2003
dc.departmentEge Üniversitesien_US
dc.description.abstractObjectives. The purpose of this study was to develop a reproducible, quantitative model of Candida albicans adhesion to human dentin through the use of a colorimetric method and to evaluate the effect of smear layer on candidal adhesion. Study design. Dentin disks with or without smear layer were incubated with C albicans (10(8) cells/mL) for 4 hours. After incubation, the disks were exposed to an (2,3)-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)-carbonyl]-2H-tetrazolium hydroxide-coenzyme Q solution for 2 hours. The color of (2,3)-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)-carbonyl]-2H-tetrazolium hydroxide formazan in the supernatant was determined spectrophotometrically at 492 nm. To relate formazan formation to cell numbers, standard curves were generated with known numbers of yeast cells without dentin. The number of adherent cells per square millimeter was then calculated. Results. The number of attached C albicans cells was 2.4 X 10(4) per square millimeter in dentin with smear layer and 1.5 X 10(4) in dentin without smear layer (P < .05). Conclusion. (2,3)-Bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)-carbonyl]-2H-tetrazolium hydroxide assay is a potential microbiologic tool for the quantitative determination of Candida adhesion to human dentin.en_US
dc.identifier.doi10.1016/S1079-2104(03)00165-3en_US
dc.identifier.endpage206en_US
dc.identifier.issn1079-2104
dc.identifier.issue2en_US
dc.identifier.pmid12931094en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage201en_US
dc.identifier.urihttps://doi.org/10.1016/S1079-2104(03)00165-3
dc.identifier.urihttps://hdl.handle.net/11454/37682
dc.identifier.volume96en_US
dc.identifier.wosWOS:000184766700013en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMosby, Incen_US
dc.relation.ispartofOral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodonticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleA new method for studying the adhesion of Candida albicans to dentin in the presence or absence of smear layeren_US
dc.typeArticleen_US

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