Expression profiling of RE1-silencing transcription factor (REST), REST corepressor 1 (RCOR1), and Synapsin 1 (SYN1) genes in human gliomas

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dc.contributor.authorYucebas, Musteyde
dc.contributor.authorSusluer, Sunde Yilmaz
dc.contributor.authorCaglar, Hasan Onur
dc.contributor.authorBalci, Tugce
dc.contributor.authorSigva, Z. Ozlem Dogan
dc.contributor.authorAkalin, Taner
dc.contributor.authorOktar, Nezih
dc.contributor.authorDalbasti, Tayfun
dc.contributor.authorAvci, Cigir Biray
dc.contributor.authorGunduz, Cumhur
dc.date.accessioned2019-10-27T23:09:14Z
dc.date.available2019-10-27T23:09:14Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractPurpose: The repressor element 1 (RE-1) silencing transcription factor (REST) is a transcription factor which represses the expression of neuronal differentiation-related genes including SYN1 gene. CoREST, encoded by RCOR1 gene, binds to the REST protein for remodeling of chromatin structure. Although there is a relation among REST, RCOR1, and SYN1 genes, the role of these genes in glioma tumors is still unclear. In this study, expressions of REST, RCOR1, and SYN1 genes were detected in primary cultures derived from tumor samples of diffuse astrocytoma (DA), anaplastic oligodendroglioma (AO), and glioblastoma multiforme (GBM) cases. Methods: Expression profiles were analysed by RT-qPCR and the copy number variations were examined with qPCR in primary cultures. ChIP assay was performed to show binding characteristics of REST and CoREST proteins on promoter region of SYN1 gene. Results: Means of relative expression for REST were as follows: 0.7898, 0.7606, and 0.7318 in DA, AO, and GBM groups, respectively. For RCOR1, expression means in DA, AO, and GBM groups were 0.7203, 0.7334, and 0.7230, respectively. SYN1 expression means were as follows: 0.3936, 0.3192, and 0.3197 in DA, AO, and GBM groups, respectively. Neither gain nor loss of copy numbers were detected for REST and RCOR1 genes in all groups. Copy loss for SYN1 was detected in primary culture of a DA case. REST and CoREST presented positive precipitation pattern on promoter region of SYN1 gene. Conclusions: Expressions of REST and RCOR1 genes may downregulate SYN1 expression in gliomas. Low expression pattern of SYN1 may maintain cancer stem-like phenotype which contributes to development of gliomas.en_US
dc.description.sponsorshipEge University Medical Faculty Research Project SubcommitteeEge University [APAK 10-TIP-080]en_US
dc.description.sponsorshipThis study was supported by Ege University Medical Faculty Research Project Subcommittee (Grant number: APAK 10-TIP-080).en_US
dc.identifier.endpage972en_US
dc.identifier.issn1107-0625
dc.identifier.issn2241-6293
dc.identifier.issue4en_US
dc.identifier.pmid27685921en_US
dc.identifier.startpage964en_US
dc.identifier.urihttps://hdl.handle.net/11454/52571
dc.identifier.volume21en_US
dc.identifier.wosWOS:000383306700027en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherImprimatur Publicationsen_US
dc.relation.ispartofJournal of Buonen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectgene expressionen_US
dc.subjectgliomasen_US
dc.subjectRCOR1en_US
dc.subjectRESTen_US
dc.subjectSYN1en_US
dc.titleExpression profiling of RE1-silencing transcription factor (REST), REST corepressor 1 (RCOR1), and Synapsin 1 (SYN1) genes in human gliomasen_US
dc.typeArticleen_US

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