Photoinduced in situ formation of clickable PEG hydrogels and their antibody conjugation
Küçük Resim Yok
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A simple approach for the preparation of a clickable poly(ethylene glycol)-based hydrogel as a polymeric support for protein immobilization via photoinitiated free radical polymerization of poly(ethylene glycol) diacrylate and propargyl acrylate is established. Bioconjugation to the obtained gels was achieved by azide-alkyne click reaction with azido-functionalized anti-immunoglobulin G (anti-IgG) and anti-His tag antibodies. Evaluation of the affinities of the PEG hydrogels to the corresponding substrates (IgG and His-tagged YFP, respectively) indicates their specific binding capability.
Açıklama
Anahtar Kelimeler
protein adsorption, click chemistry, bioconjugation, photopolymerization, hydrogels
Kaynak
Designed Monomers and Polymers
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
18
Sayı
2
