* Concordance in molecular genetic analysis of tumour tissue, plasma, and exhaled breath condensate samples from lung cancer patients

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dc.contributor.authorVardarli, Asli Tetik
dc.contributor.authorPelit, Levent
dc.contributor.authorAldag, Ceyda
dc.contributor.authorKorba, Korcan
dc.contributor.authorCelebi, Caglar
dc.contributor.authorDizdas, Tugberk Nail
dc.contributor.authorGoksel, Tuncay
dc.date.accessioned2020-12-01T11:59:16Z
dc.date.available2020-12-01T11:59:16Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractAim. Lung adenocarcinoma is characterized by poor prognosis and short survival rates. Therefore, tools to identify the tumoural molecular structure and guide effective diagnosis and therapy decisions are essential. Surgical biopsies are highly invasive and not conducive for patient follow-up. To better understand disease prognosis, novel non-invasive analytic methods are needed. the aim of the present study is to identify the genetic mutations in formalin-fixed paraffin-embedded (FFPE) tissue, plasma, and exhaled breath condensate (EBC) samples by next-generation sequencing and evaluate their utility in the diagnosis and follow-up of patients with lung adenocarcinoma. Method. FFPE, plasma, and EBC samples were collected from 12 lung adenocarcinoma patients before treatment. DNA was extracted from the specimens using an Invitrogen PureLink Genomic DNA Kit according to the manufacturer's instructions. Amplicon-based sequencing was performed using Ion AmpliSeq Colon and Lung Cancer Research Panel v2. Results. Genetic alterations were detected in all FFPE, plasma, and EBC specimens. the mutations in PIK3CA, MET, PTEN, SMAD4, and FGFR2 genes were highly correlated in six patients. Somatic and novel mutations detected in tissue and EBC samples were highly correlated in one additional patient. the EGFR p.L858R and KRAS p.G12C driver mutations were found in both the FFPE tissue specimens and the corresponding EBC samples of the lung adenocarcinoma patients. Conclusion. the driver mutations were detected in EBC samples from lung adenocarcinoma patients. the analysis of EBC samples represents a promising non-invasive method to detect mutations in lung cancer and guide diagnosis and follow-up.en_US
dc.description.sponsorshipScientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TUBITAK-1003-216S591, 216S435]en_US
dc.description.sponsorshipThe research project was supported by the Scientific and Technological Research Council of Turkey (TUBITAK-1003-216S591 and 216S435).en_US
dc.identifier.doi10.1088/1752-7163/ab739ben_US
dc.identifier.issn1752-7155
dc.identifier.issn1752-7163
dc.identifier.issue3en_US
dc.identifier.pmid32031993en_US
dc.identifier.scopus2-s2.0-85083544794en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1088/1752-7163/ab739b
dc.identifier.urihttps://hdl.handle.net/11454/62217
dc.identifier.volume14en_US
dc.identifier.wosWOS:000528569000001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherIop Publishing Ltden_US
dc.relation.ispartofJournal of Breath Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectdriver mutationsen_US
dc.subjectlung adenocarcinomaen_US
dc.subjectexhaled breath condensateen_US
dc.subjectvolatile biopsyen_US
dc.title* Concordance in molecular genetic analysis of tumour tissue, plasma, and exhaled breath condensate samples from lung cancer patientsen_US
dc.typeArticleen_US

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