Variability of Isoquinoline Alkaloid Profiles and Anticholinesterase Activities with Binding-Mode Predictions of Glaucium flavum Population

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dc.authoridCoban, Gunes/0000-0003-4512-4392
dc.authoridBOZKURT, BUKET/0000-0003-2858-5162
dc.contributor.authorBozkurt, Buket
dc.contributor.authorUlkar, Duygu
dc.contributor.authorNurlu, Necati
dc.contributor.authorCoban, Gunes
dc.contributor.authorGumus, Zinar Pinar
dc.contributor.authorUnver-Somer, Nehir
dc.date.accessioned2024-08-31T07:48:28Z
dc.date.available2024-08-31T07:48:28Z
dc.date.issued2024
dc.departmentEge Üniversitesien_US
dc.description.abstractIn this study, phytochemical and biological activity studies supported by docking were carried out on a species of the genus Glaucium, a repository of isoquinoline alkaloids. The GC-MS (Gas Chromatography-Mass Spectrometry) method is used to characterize the isoquinoline alkaloids of Glaucium flavum Crantz. (Papaveraceae). G. flavum was collected from seven different regions of Turkiye (Antalya, Urla-Izmir, Mordogan-Izmir, Mugla, Assos-Canakkale, Karabiga-Canakkale, Giresun) and totally 17 compounds were detected by GC-MS. Glaucine was found to be the major constituent in the sample collected from Mugla, whereas isocorydine was recorded to be the principal alkaloid in other samples. Further fractionation studies on G. flavum collected from Antalya province in Southwestern Turkiye, yielded five major alkaloids (isocorydine 1, dihydrosanguinarine 2, glaucine 3, dehydroglaucine 4, protopine 5) which were characterized by spectroscopic methods. Anticholinesterase activities of the extracts and isolated alkaloids were also tested by in vitro Ellman method. The isolated compounds were also analyzed by a molecular docking technique to determine the binding orientations in the gorge of the active site of acetylcholinesterase (AChE) and a homology model of butyrylcholinesterase (BuChE). This is the first comparative investigation of the phytochemical composition and biodiversity of Glaucium flavum species growing in Turkiye.en_US
dc.description.sponsorshipTUBITAK (Turkiye's Scientific and Technological Research Council) [315S064]; Ege University Science and Technology Application and Research Center (EBILTEM) [16-BIL-002]; Ege University-Scientific Research Projects (EUE-BAP) [17-ECZ-003]en_US
dc.description.sponsorshipThis research was funded by TUBITAK (Turkiye's Scientific and Technological Research Council) under project number 315S064 and Ege University Science and Technology Application and Research Center (EBILTEM) under project number 16-BIL-002 and Ege University-Scientific Research Projects (EUE-BAP) (Project no: 17-ECZ-003). The authors would like to thank Professor M. Ali Onur and G. Irem Kaya for their assistance in collecting the plants, as well as FABAL (Ege University Faculty of Pharmacy Pharmaceutical Sciences Research Centre) for facilitating the GC-MS analysis.en_US
dc.identifier.doi10.1002/cbdv.202301865
dc.identifier.issn1612-1872
dc.identifier.issn1612-1880
dc.identifier.issue4en_US
dc.identifier.pmid38415909en_US
dc.identifier.scopus2-s2.0-85187653399en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1002/cbdv.202301865
dc.identifier.urihttps://hdl.handle.net/11454/104799
dc.identifier.volume21en_US
dc.identifier.wosWOS:001185550900001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofChemistry & Biodiversityen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240831_Uen_US
dc.subjectGlaucium Flavumen_US
dc.subjectIsoquinoline Alkaloidsen_US
dc.subjectGc-Msen_US
dc.subjectAnticholinesterase Activityen_US
dc.subjectMolecular Dockingen_US
dc.titleVariability of Isoquinoline Alkaloid Profiles and Anticholinesterase Activities with Binding-Mode Predictions of Glaucium flavum Populationen_US
dc.typeArticleen_US

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