Effect of fish-oil enriched diet on leukotriene level in experimental trinitrobenzen sulfonic acid (TNB) colitis in rats [Deneysel trinitrobenzensulfonik asit (TNB) kolitinde balik yagindan zengin diyetin lokotrien duzeylerine etkisi]
Küçük Resim Yok
Tarih
1997
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The etiology of inflammatory bowel disease (IBD) is still unknown and the treatment of recurrences of IBD is also inadequate. In this study, we investigated the protective role of a fish oil (FO) - enriched diet in a rat model of TNP colitis. 20 male Wistar-Albino rats were randomized into 2 groups (n = 10). All of them were fed for 6 weeks with a standard diet (group-1) and standard diet plus FO (% 8) (group-2). At the end of this period, TNP (30 mg in 0,25 ml of % 30 ethanol) were intrarectally administered. After two weeks, the rats were sacrificed and the distal colon was removed. The Specimens were histopathologically evaluated. The MPO enzyme activities were measured and leukotriene B4 (LTB4) and LTC4 levels were determined by radioimmunoassay. MPO activities, LTB4 and LTC4 levels were (respectively) 30,41 ± 1,94 Ug/wet weight, 372,1 ± 65,24 pg/mg tissue protein, 450,0 ± 56,45 pg/mg tissue protein. These levels were reduced in group-2 (respectively) 2,43 ± 0,47 Ug/wet weight, 34,5 ± 8,16 and 64,0 ± 10,46 pg/mg tissue protein. There were significant differences in MPO activities, LTB4 and LTC4 levels and histopathological scores between the two groups (respectively p = 0,0108, p = 0,0034, p = 0,0024, p = 0,002). In conclusion, FO-enriched diet could reduce the colon damage in TNP colitis in rats.
Açıklama
Anahtar Kelimeler
fish oil, LTB4, LTC4, TNB colitis
Kaynak
Turkish Journal of Gastroenterology
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
8
Sayı
2
