Determination of mitochondrial fragmentation and autophagosome formation in C2C12 skeletal muscle cells
| dc.contributor.author | Türkseven, Saadet | |
| dc.contributor.author | Zorzano, Antonio | |
| dc.date.accessioned | 2020-12-01T12:23:17Z | |
| dc.date.available | 2020-12-01T12:23:17Z | |
| dc.date.issued | 2013 | |
| dc.department | Ege Üniversitesi | en_US |
| dc.description.abstract | Aim: To investigate the effect of carbonyl cyanide 3-chlorophenylhydrazone (CCCP) on mitochondrial elimination in C2C12 skeletal muscle cells. Materials and methods: C2C12 muscle cells stably expressing Mito-DsRed were treated with DMSO or CCCP at the doses of 10, 20, and 30 µM for 3, 6, and 24 h. After specific treatments, cells were fixed in 4% paraformaldehyde and immunocytochemistry assays were performed using appropriate antibodies to evaluate mitochondrial morphology and autophagosome formation. Total cell lysates were used for western blot assays. Results: CCCP induces mitochondrial fragmentation in C2C12 cells. in response to a mitochondrial uncoupler, endogenous autophagosome formation is significantly increased (P < 0.001). Co-localization analyses of confocal images indicate that fragmented mitochondria undergo engulfment by autophagosomes. Conclusion: the findings of this study will enhance our understanding of the mitophagic process in C2C12 skeletal muscle cells in order to identify molecular mechanisms governing removal of dysfunctional mitochondria. | en_US |
| dc.identifier.endpage | 781 | en_US |
| dc.identifier.issn | 1300-0144 | |
| dc.identifier.issn | 1303-6165 | |
| dc.identifier.issue | 5 | en_US |
| dc.identifier.startpage | 775 | en_US |
| dc.identifier.uri | https://app.trdizin.gov.tr//makale/TWpFeE56QXhNUT09 | |
| dc.identifier.uri | https://hdl.handle.net/11454/65184 | |
| dc.identifier.volume | 43 | en_US |
| dc.indekslendigikaynak | TR-Dizin | en_US |
| dc.language.iso | en | en_US |
| dc.relation.ispartof | Turkish Journal of Medical Sciences | en_US |
| dc.relation.publicationcategory | Makale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | Cerrahi | en_US |
| dc.title | Determination of mitochondrial fragmentation and autophagosome formation in C2C12 skeletal muscle cells | en_US |
| dc.type | Article | en_US |
