Alkaline Phosphatase Based Amperometric Biosensor Immobilized by Cysteamine-Glutaraldehyde Modified Self-Assembled Monolayer

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dc.contributor.authorYorganci, Emine
dc.contributor.authorAkyilmaz, Erol
dc.date.accessioned2019-10-27T21:36:11Z
dc.date.available2019-10-27T21:36:11Z
dc.date.issued2011
dc.departmentEge Üniversitesien_US
dc.description.abstractAlkaline phosphatase (ALP) was immobilized with cross-linking agents glutaraldehyde and cysteamine by forming a self-assembled monolayer on a screen printed gold electrode. ALP converts p-nitrophenyl phosphate to p-nitrophenol and phosphate. p-Nitrophenol loses H(broken vertical bar) ion and turns into the negatively charged compound p-nitrophenolate at medium pH. As a result, the unstable product formed is measured chronoamperometrically at an application potential of + 0.95 V. The biosensor response depends linearly on p-nitrophenyl phosphate concentration between 0.05 - 0.6 mM with a response time of 40 seconds. Detection limit of the biosensor is 0.033 mM.en_US
dc.description.sponsorshipEge UniversityEge University [2009 FEN 008]en_US
dc.description.sponsorshipThis project was found by Ege University Research Found (Project No: 2009 FEN 008).en_US
dc.identifier.doi10.3109/10731199.2011.563363en_US
dc.identifier.endpage323en_US
dc.identifier.issn1073-1199
dc.identifier.issue5en_US
dc.identifier.pmid21663400en_US
dc.identifier.startpage317en_US
dc.identifier.urihttps://doi.org/10.3109/10731199.2011.563363
dc.identifier.urihttps://hdl.handle.net/11454/46008
dc.identifier.volume39en_US
dc.identifier.wosWOS:000294740300009en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherInforma Healthcareen_US
dc.relation.ispartofArtificial Cells Blood Substitutes and Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectalkaline phosphatiseen_US
dc.subjectbiosensoren_US
dc.subjectself-assembled monolayeren_US
dc.subjectamperometryen_US
dc.subjectp-nitrophenyl phosphateen_US
dc.titleAlkaline Phosphatase Based Amperometric Biosensor Immobilized by Cysteamine-Glutaraldehyde Modified Self-Assembled Monolayeren_US
dc.typeArticleen_US

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